Automated real-time PCR to determine K-ras codon 12 mutations in non-small cell lung cancer: Comparison with immunohistochemistry and clinico-pathological features

  • Authors:
    • Wolfgang Hilbe
    • Margit Dlaska
    • Hans-Christoph Duba
    • Stephan Dirnhofer
    • Wolfgang Eisterer
    • Frieda Oberwasserlechner
    • Andreas Mildner
    • Thomas Schmid
    • Thomas Kühr
    • Ewald Wöll
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.1121
  • Pages: 1121-1126
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Abstract

A real-time PCR technique with automated computerized analysis (TaqMan™) was tested to detect K-ras mutations in 66 patients suffering from NSCLC. This technology is characterized by high reproducibility of data and a time-saving analysis procedure. In 11% (7/66) of the tumour specimens and 2% (1/58) of adjacent tumour-free lung specimens a K-ras codon 12 mutation was detected. In adenocarcinomas containing ≥40% tumour cells, however, K-ras mutations were seen in 25% of the cases. The point mutations detected in tumours were GGT↷TGT in five cases and GGT↷GTT in two cases. As compared with immunohistochemical parameters, the K-ras mutated group was characterized by a c-erbB-2 negativity (p=0.04) and a smaller number of c-erbB-3 (p=0.02) positive cases. EGFR, bcl-2, p53, Ki-67 and p120 expression did not differ significantly. Determination of the K-ras point mutations by automated TaqMan PCR in NSCLC tumour specimens is feasable and highly specific. Due to its high throughput capacity this method represents a valuable tool for routine screening.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hilbe W, Dlaska M, Duba H, Dirnhofer S, Eisterer W, Oberwasserlechner F, Mildner A, Schmid T, Kühr T, Wöll E, Wöll E, et al: Automated real-time PCR to determine K-ras codon 12 mutations in non-small cell lung cancer: Comparison with immunohistochemistry and clinico-pathological features. Int J Oncol 23: 1121-1126, 2003
APA
Hilbe, W., Dlaska, M., Duba, H., Dirnhofer, S., Eisterer, W., Oberwasserlechner, F. ... Wöll, E. (2003). Automated real-time PCR to determine K-ras codon 12 mutations in non-small cell lung cancer: Comparison with immunohistochemistry and clinico-pathological features. International Journal of Oncology, 23, 1121-1126. https://doi.org/10.3892/ijo.23.4.1121
MLA
Hilbe, W., Dlaska, M., Duba, H., Dirnhofer, S., Eisterer, W., Oberwasserlechner, F., Mildner, A., Schmid, T., Kühr, T., Wöll, E."Automated real-time PCR to determine K-ras codon 12 mutations in non-small cell lung cancer: Comparison with immunohistochemistry and clinico-pathological features". International Journal of Oncology 23.4 (2003): 1121-1126.
Chicago
Hilbe, W., Dlaska, M., Duba, H., Dirnhofer, S., Eisterer, W., Oberwasserlechner, F., Mildner, A., Schmid, T., Kühr, T., Wöll, E."Automated real-time PCR to determine K-ras codon 12 mutations in non-small cell lung cancer: Comparison with immunohistochemistry and clinico-pathological features". International Journal of Oncology 23, no. 4 (2003): 1121-1126. https://doi.org/10.3892/ijo.23.4.1121