In situ detection of telomeres by fluorescence in situ hybridization and telomerase activity in glioblastoma multiforme: Correlation with p53 status, EGFR, c-myc, MIB1, and Topoisomerase IIα protein expression

  • Authors:
    • Clelia Miracco
    • M. Margherita De Santi
    • Pietro Luzi
    • Anna Vittoria Lalinga
    • Lorella Laurini
    • Maria Caterina De Nisi
    • Giuseppina Angeloni
    • Marco Brogi
    • Concetta Cardone
    • Antonietta Carducci
    • Felice Arcuri
    • Piero Tosi
    • Giovanni Rubino
    • Luigi Pirtoli
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijo.23.6.1529
  • Pages: 1529-1535
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Abstract

Aberrations of genes/proteins regulating cell cycle and growth, increased proliferation and telomerase activity (TA) are documentable in glioblastoma multiforme. TA is more frequently detectable in secondary glioblastoma, which is also characterized by p53 mutation/overexpression. Discordant telomere (Te) length values have been reported in glioblastomas with and without TA. In 31 glioblastomas, in which pre-existing astrocytoma was not documented, we compared cases with and without TA for the expression of p53, EGFR, c-Myc, MIB-1 and Topoisomerase IIα; p53 mutations were also investigated by SSCP-PCR. Correlations were made with Te parameters [TePs: number (TeNo), length and area] as evaluated by image analysis in interphase nuclei of fluorescence in situ hybridization (FISH)-processed sections. We found no differences in the expression of the proteins evaluated and in TePs, except Te/nuclear area %, which was significantly lower in TA+ cases (p=0.02). TePs were, instead, inversely correlated with TA (p=0.0001). TA was positively correlated with MIB1 staining index in the TA+ cases (p=0.033), which also showed a positive correlation between TeNo and EGFR expression (p=0.042), and a trend towards a negative correlation between TeNo and p53 expression (p=0.05). Tumors overexpressing EGFR had a significantly shorter lifetime (p=0.0001). TeNo seems to be inversely correlated to tumor proliferation and lifetime in glioblastoma multiforme.

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December 2003
Volume 23 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Miracco C, De Santi MM, Luzi P, Lalinga AV, Laurini L, De Nisi MC, Angeloni G, Brogi M, Cardone C, Carducci A, Carducci A, et al: In situ detection of telomeres by fluorescence in situ hybridization and telomerase activity in glioblastoma multiforme: Correlation with p53 status, EGFR, c-myc, MIB1, and Topoisomerase IIα protein expression. Int J Oncol 23: 1529-1535, 2003
APA
Miracco, C., De Santi, M.M., Luzi, P., Lalinga, A.V., Laurini, L., De Nisi, M.C. ... Pirtoli, L. (2003). In situ detection of telomeres by fluorescence in situ hybridization and telomerase activity in glioblastoma multiforme: Correlation with p53 status, EGFR, c-myc, MIB1, and Topoisomerase IIα protein expression. International Journal of Oncology, 23, 1529-1535. https://doi.org/10.3892/ijo.23.6.1529
MLA
Miracco, C., De Santi, M. M., Luzi, P., Lalinga, A. V., Laurini, L., De Nisi, M. C., Angeloni, G., Brogi, M., Cardone, C., Carducci, A., Arcuri, F., Tosi, P., Rubino, G., Pirtoli, L."In situ detection of telomeres by fluorescence in situ hybridization and telomerase activity in glioblastoma multiforme: Correlation with p53 status, EGFR, c-myc, MIB1, and Topoisomerase IIα protein expression". International Journal of Oncology 23.6 (2003): 1529-1535.
Chicago
Miracco, C., De Santi, M. M., Luzi, P., Lalinga, A. V., Laurini, L., De Nisi, M. C., Angeloni, G., Brogi, M., Cardone, C., Carducci, A., Arcuri, F., Tosi, P., Rubino, G., Pirtoli, L."In situ detection of telomeres by fluorescence in situ hybridization and telomerase activity in glioblastoma multiforme: Correlation with p53 status, EGFR, c-myc, MIB1, and Topoisomerase IIα protein expression". International Journal of Oncology 23, no. 6 (2003): 1529-1535. https://doi.org/10.3892/ijo.23.6.1529