Transforming growth factor-β is an endogenous radioresistance factor in the esophageal adenocarcinoma cell line OE-33

  • Authors:
    • Albert H. Kim
    • Deborah A. Lebman
    • Christopher M. Dietz
    • Steven R. Snyder
    • Kerry W. Eley
    • Theodore D. Chung
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijo.23.6.1593
  • Pages: 1593-1599
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Abstract

Transforming growth factor (TGF)-β has profound effects on epithelial cell differentiation and is capable of modulating the response to exposure to ionizing radiation. We recently reported that TGF-β downregulates c-myc mRNA expression and inhibits the growth of OE-33 esophageal carcinoma cells in vitro. These studies investigate the role of TGF-β in the in vitro radiation response of OE-33 and four other human esophageal cancer cell lines. TGF-β enhanced radioresistance of OE-33 cells, but did not affect the radiosensitivity of either of the two other adenocarcinoma cell lines BIC1 and SEG1 or of squamous carcinomas KYSE and OE-21. The TGF-β enhanced radioresistance phenotype was associated with induced G0/G1 cell cycle arrest and upregulation of the G1 cyclin-dependent kinase inhibitor p27kip1 as well as downregulation of c-myc protein expression. Comparison of the relative radiosensitivities of untreated cells suggested that OE-33 (SF2 = 0.71) cells were inherently more radioresistant than BIC1 or SEG1 cells (SF2 = 0.6 and 0.56, respectively). Conditioned medium obtained from unirradiated OE-33 cells enhanced radioresistance compared with fresh medium. This enhancement was abrogated by preincubation of conditioned medium with a neutralizing anti-TGF-β antibody suggesting endogenous TGF-β production by OE-33 cells. Enzyme-linked immunoabsorbent assays revealed that exposure to ionizing radiation increased TGF-β production in all five cell lines. These results suggest that TGF-β acts as an endogenous, radiation-inducible radioresistance factor in OE-33 esophageal carcinoma cells.

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December 2003
Volume 23 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim AH, Lebman DA, Dietz CM, Snyder SR, Eley KW and Chung TD: Transforming growth factor-β is an endogenous radioresistance factor in the esophageal adenocarcinoma cell line OE-33. Int J Oncol 23: 1593-1599, 2003
APA
Kim, A.H., Lebman, D.A., Dietz, C.M., Snyder, S.R., Eley, K.W., & Chung, T.D. (2003). Transforming growth factor-β is an endogenous radioresistance factor in the esophageal adenocarcinoma cell line OE-33. International Journal of Oncology, 23, 1593-1599. https://doi.org/10.3892/ijo.23.6.1593
MLA
Kim, A. H., Lebman, D. A., Dietz, C. M., Snyder, S. R., Eley, K. W., Chung, T. D."Transforming growth factor-β is an endogenous radioresistance factor in the esophageal adenocarcinoma cell line OE-33". International Journal of Oncology 23.6 (2003): 1593-1599.
Chicago
Kim, A. H., Lebman, D. A., Dietz, C. M., Snyder, S. R., Eley, K. W., Chung, T. D."Transforming growth factor-β is an endogenous radioresistance factor in the esophageal adenocarcinoma cell line OE-33". International Journal of Oncology 23, no. 6 (2003): 1593-1599. https://doi.org/10.3892/ijo.23.6.1593