Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells

  • Authors:
    • I. B. Appelskog
    • O. Ammerpohl
    • I. G. Svechnikova
    • W.-O. Lui
    • P. M. Almqvist
    • T. J. Ekström
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/ijo.24.6.1419
  • Pages: 1419-1425
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Abstract

The histone deacetylase (HDAC) inhibitor 4-phenylbutyrate (4-PB) is a non-toxic compound that can induce differentiation and promote maturation of various types of malignant cells. In the present study we show that 4-PB inhibit glioma cell proliferation, induce apoptosis and decrease mRNA expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in a concentration-dependent manner. Proliferation of established rat glioma cell lines (RG2 and C6) in culture was significantly decreased after treatment with 4-PB (2-40 mM). Low concentrations of 4-PB (2-20 mM) induced cell differentiation followed by apoptosis, whereas higher concentrations of 4-PB (40 mM) induced cell necrosis. Also, low concentrations of 4-PB significantly decreased GAPDH mRNA expression in C6 and RG2 rat glioma cells, suggesting a link between decreased cell proliferation, energy consumption, and down-regulation of GAPDH gene expression. We have found that GAPDH mRNA expression is markedly increased in human glioblastoma tissues. Therefore, the novel effect of 4-PB described here may offer means to suppress growth of glioma cells by diminishing the key reaction in glycolysis as a therapeutic approach for cancer.

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June 2004
Volume 24 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Appelskog IB, Ammerpohl O, Svechnikova IG, Lui W, Almqvist PM and Ekström TJ: Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells. Int J Oncol 24: 1419-1425, 2004
APA
Appelskog, I.B., Ammerpohl, O., Svechnikova, I.G., Lui, W., Almqvist, P.M., & Ekström, T.J. (2004). Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells. International Journal of Oncology, 24, 1419-1425. https://doi.org/10.3892/ijo.24.6.1419
MLA
Appelskog, I. B., Ammerpohl, O., Svechnikova, I. G., Lui, W., Almqvist, P. M., Ekström, T. J."Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells". International Journal of Oncology 24.6 (2004): 1419-1425.
Chicago
Appelskog, I. B., Ammerpohl, O., Svechnikova, I. G., Lui, W., Almqvist, P. M., Ekström, T. J."Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells". International Journal of Oncology 24, no. 6 (2004): 1419-1425. https://doi.org/10.3892/ijo.24.6.1419