Growth arrest and apoptosis in adult T cell leukemia cell lines following IL-2 deprivation

  • Authors:
    • Satoshi Fujimura
    • Fumiko Arakawa
    • Yasuaki Yamada
    • Shaoxi Liao
    • Pranay D. Khare
    • Masahide Kuroki
    • Junko Ono
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijo.25.2.437
  • Pages: 437-443
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Abstract

Adult T-cell leukemia (ATL) is a peripheral T-cell neoplasm caused by human T-cell leukemia virus type-I (HTLV-I). Since ATL cells often require IL-2 for their proliferation and survival, we examined the effect of IL-2 deprivation on the IL-2-dependent ATL cells established from ATL patients. After IL-2 withdrawal, these cells were arrested in the G1 phase and then underwent apoptosis. p27Kip1 was observed to act as a cell cycle inhibitor. A decrease in the amount of Bcl-xL was more distinct than that of Bcl-2, while Bax increased slightly during IL-2 withdrawal. The activation of caspase-3 and the loss of mitochondrial membrane potential were also observed. An overexpression of Bcl-xL protein in the KK1, one of the ATL cell lines, suppressed apoptosis by the 3rd day, however, apoptosis could not be prevented completely. Thereafter, a decrease in Bcl-xL and an activation of caspase-3 were observed even under the overexpression of Bcl-xL. The mitochondrial membrane potential and the intra-cellular levels of reactive oxygen species (ROS) also changed due to IL-2 deprivation. From these results, the IL-2 signals are considered to be essential for the survival of ATL cells, and the interruption of IL-2 signaling might thus be useful as a potentially new treatment for ATL.

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August 2004
Volume 25 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Fujimura S, Arakawa F, Yamada Y, Liao S, Khare PD, Kuroki M and Ono J: Growth arrest and apoptosis in adult T cell leukemia cell lines following IL-2 deprivation. Int J Oncol 25: 437-443, 2004
APA
Fujimura, S., Arakawa, F., Yamada, Y., Liao, S., Khare, P.D., Kuroki, M., & Ono, J. (2004). Growth arrest and apoptosis in adult T cell leukemia cell lines following IL-2 deprivation. International Journal of Oncology, 25, 437-443. https://doi.org/10.3892/ijo.25.2.437
MLA
Fujimura, S., Arakawa, F., Yamada, Y., Liao, S., Khare, P. D., Kuroki, M., Ono, J."Growth arrest and apoptosis in adult T cell leukemia cell lines following IL-2 deprivation". International Journal of Oncology 25.2 (2004): 437-443.
Chicago
Fujimura, S., Arakawa, F., Yamada, Y., Liao, S., Khare, P. D., Kuroki, M., Ono, J."Growth arrest and apoptosis in adult T cell leukemia cell lines following IL-2 deprivation". International Journal of Oncology 25, no. 2 (2004): 437-443. https://doi.org/10.3892/ijo.25.2.437