Overexpression and amplification of STK15 in human gliomas

  • Authors:
    • Alexandra Klein
    • Wilfried Reichardt
    • Volker Jung
    • Klaus D. Zang
    • Eckart Meese
    • Steffi Urbschat
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijo.25.6.1789
  • Pages: 1789-1794
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Abstract

The serine/threonine kinase 15 (STK15) at chromosome 20q13.2 is frequently shown to be amplified and overexpressed in several human cancers. STK15 has been reported to act as a cell cycle regulator and its overexpression induces centrosome amplification and aneuploidy. Recently we showed that STK15 even plays a role in human malignant brain tumours and we described an amplification of the gene in 31% of the investigated gliomas. In this study we scrutinized the correlation of increased STK15 on DNA and mRNA levels in gliomas of different histological grades. Southern blotting confirmed the amplification frequency of the STK15 gene, which had been previously detected by comparative PCR. In total, DNA gains were found in 26% of the investigated gliomas. Interestingly, we detected overexpression of STK15 mRNA in 60% of the analyzed brain tumours. The elevated expression does not strongly correlate with gains on DNA level, but all cases with an amplification of the STK15 gene display overexpression. Gains of the STK15 gene seem to occur irrespectively of the histological grades of the tumours, so that STK15 probably is not a progression associated factor. Amplification and overexpression of the kinase rather represent a primary alteration in human gliomas, which could play an important role as an early event in all glioma subtypes.

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December 2004
Volume 25 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Klein A, Reichardt W, Jung V, Zang KD, Meese E and Urbschat S: Overexpression and amplification of STK15 in human gliomas. Int J Oncol 25: 1789-1794, 2004
APA
Klein, A., Reichardt, W., Jung, V., Zang, K.D., Meese, E., & Urbschat, S. (2004). Overexpression and amplification of STK15 in human gliomas. International Journal of Oncology, 25, 1789-1794. https://doi.org/10.3892/ijo.25.6.1789
MLA
Klein, A., Reichardt, W., Jung, V., Zang, K. D., Meese, E., Urbschat, S."Overexpression and amplification of STK15 in human gliomas". International Journal of Oncology 25.6 (2004): 1789-1794.
Chicago
Klein, A., Reichardt, W., Jung, V., Zang, K. D., Meese, E., Urbschat, S."Overexpression and amplification of STK15 in human gliomas". International Journal of Oncology 25, no. 6 (2004): 1789-1794. https://doi.org/10.3892/ijo.25.6.1789