Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro

  • Authors:
    • Mika Kijima
    • Masanao Saio
    • Guan-Feng Oyang
    • Tatsuhiko Suwa
    • Rumiko Miyauchi
    • Yasushi Kojima
    • Hisashi Imai
    • Jiro Nakagawa
    • Kenichi Nonaka
    • Naoki Umemura
    • Takashi Nishimura
    • Tsuyoshi Takami
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.679
  • Pages: 679-684
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Abstract

The natural killer (NK) cell is one of the key cells in discriminating major histocompatibility complex (MHC) negative ‘missing-self’ target tumor cells, and interleukin 2 (IL-2) treatment was effective in inducing NK cell activation. In this study, we tried to clarify how poorly-immunogenic murine B16 melanoma could be discriminated in vivo by creating an IL-2 cDNA-transduced immunogene therapy model (B16/IL-2). In vitro study showed that IL-2 introduction did not induce MHC class I. However, immune cells depleted total tumor digest, which consisted of 90% anti-melanoma MM2-9B6-positive cells that revealed B16/IL-2 strongly, and control tumor cells (B16/mock) partially expressed MHC class I in vivo. In the B16/IL-2 model, NK cell infiltration was 10 times higher than B16/mock (7.6 versus 0.73, p=0.017). In addition, the cell surface of CD69-expressing NK cell population was increased in B16/IL-2, and the interferon gamma (IFNγ) message level in NK cells was significantly increased in B16/IL-2 (p=0.0359). Interestingly, NK cell depletion in vivo completely abolished MHC class I expression on B16/mock, and decreased MHC class I expression and T-cell infiltration in B16/IL-2. These data suggest that NK cells are not only important for missing-self recognition, but are also crucial for induction of tumor cell MHC molecule expression and play an important role in helping acquired immunity to recognize tumor cells.

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March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kijima M, Saio M, Oyang G, Suwa T, Miyauchi R, Kojima Y, Imai H, Nakagawa J, Nonaka K, Umemura N, Umemura N, et al: Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro. Int J Oncol 26: 679-684, 2005
APA
Kijima, M., Saio, M., Oyang, G., Suwa, T., Miyauchi, R., Kojima, Y. ... Takami, T. (2005). Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro. International Journal of Oncology, 26, 679-684. https://doi.org/10.3892/ijo.26.3.679
MLA
Kijima, M., Saio, M., Oyang, G., Suwa, T., Miyauchi, R., Kojima, Y., Imai, H., Nakagawa, J., Nonaka, K., Umemura, N., Nishimura, T., Takami, T."Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro". International Journal of Oncology 26.3 (2005): 679-684.
Chicago
Kijima, M., Saio, M., Oyang, G., Suwa, T., Miyauchi, R., Kojima, Y., Imai, H., Nakagawa, J., Nonaka, K., Umemura, N., Nishimura, T., Takami, T."Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro". International Journal of Oncology 26, no. 3 (2005): 679-684. https://doi.org/10.3892/ijo.26.3.679