Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine

  • Authors:
    • Yasumasa Kato
    • Mamoru Tsukuda
    • Yoji Nagashima
    • Shinri Koshika
    • Naoki Sakai
    • Masahiro Yao
    • Yoshinobu Kubota
    • Ichiro Aoki
    • William H. Colledge
    • Jean-Michel Foidart
    • Ryu-Ichiro Hata
    • Erik W. Thompson
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/ijo.27.3.759
  • Pages: 759-768
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Abstract

SPARC (secreted protein acidic and rich in cysteine)/ osteonectin/BM-40 is a matricellular protein implicated in development, cell transformation and tumorigenesis. We have examined the role of SPARC in cell transformation induced chemically with 7,12-dimethylbenz[a]anthracene (DMBA) and 12-tetradecanoylphorbol-13-acetate (TPA) in embryonic fibroblasts and in the skin of mice. Embryonic fibroblasts from SPARCnull mice showed increases in cell proliferation, enhanced sensitivity to DMBA and a higher number of DMBA/TPA-induced transformation foci. The number of DMBA-DNA adducts was 9 times higher in SPARCnull fibroblasts and their stability was lower than wild-type fibroblasts, consistent with a reduction in excision repair cross-complementing 1 the nucleotide excision repair enzyme in these cells. The SPARCnull mice showed an increase in both the speed and number of papillomas forming after topical administration of DMBA/TPA to the skin. These papillomas showed reduced growth and reduced progression to a more malignant phenotype, indicating that the effect of SPARC on tumorigenesis depends upon the transformation stage and/or tissue context. These data reinforce a growing number of observations in which SPARC has shown opposite effects on different tumor types/stages.

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September 2005
Volume 27 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kato Y, Tsukuda M, Nagashima Y, Koshika S, Sakai N, Yao M, Kubota Y, Aoki I, Colledge WH, Foidart J, Foidart J, et al: Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine. Int J Oncol 27: 759-768, 2005
APA
Kato, Y., Tsukuda, M., Nagashima, Y., Koshika, S., Sakai, N., Yao, M. ... Thompson, E.W. (2005). Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine. International Journal of Oncology, 27, 759-768. https://doi.org/10.3892/ijo.27.3.759
MLA
Kato, Y., Tsukuda, M., Nagashima, Y., Koshika, S., Sakai, N., Yao, M., Kubota, Y., Aoki, I., Colledge, W. H., Foidart, J., Hata, R., Thompson, E. W."Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine". International Journal of Oncology 27.3 (2005): 759-768.
Chicago
Kato, Y., Tsukuda, M., Nagashima, Y., Koshika, S., Sakai, N., Yao, M., Kubota, Y., Aoki, I., Colledge, W. H., Foidart, J., Hata, R., Thompson, E. W."Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine". International Journal of Oncology 27, no. 3 (2005): 759-768. https://doi.org/10.3892/ijo.27.3.759