Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma: A new approach to identify novel tumor markers

  • Authors:
    • Yukinori Kurokawa
    • Kimi Honma
    • Ichiro Takemasa
    • Shoji Nakamori
    • Hiroko Kita-Matsuo
    • Masaaki Motoori
    • Hiroaki Nagano
    • Keizo Dono
    • Takahiro Ochiya
    • Morito Monden
    • Kikuya Kato
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/ijo.28.2.383
  • Pages: 383-391
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Abstract

Hepatocellular carcinoma (HCC) is a common malignancy, but the prognosis remains poor due to the lack of sensitive diagnostic markers. To gain insight into the central molecular features common to all types of HCC, and to identify novel diagnostic markers or therapeutic targets for HCC, we performed a gene expression profiling analysis using a high throughput RT-PCR system. After examining the mRNA expression of 3,072 genes in 204 (119 tumor and 85 non-tumor) liver samples, we identified differential gene expression between the HCV group (n=80), HBV group (n=19) and non-B, non-C group (n=20) with a principal component analysis and a correlation spectrum analysis. After selection of genes differentially expressed between tumor and non-tumor tissues (p<0.01) within each HCC group, a total of 51 differentially expressed genes (23 upregulated and 28 downregulated genes) were found to be common to the three HCC groups. Gene Ontology grouping analysis revealed that genes with functions related to cell proliferation or differentiation and genes encoding extracellular proteins were found to be significantly enriched in these 51 common genes. Using an atelocollagen-based cell transfection array for functional analysis of eight upregulated genes, five (CANX, FAM34A, PVRL2, LAMR1, and GBA) significantly inhibited cellular apoptosis by two independent assays. In conclusion, we identified 51 differentially expressed genes, common to all HCC types. Among these genes, there was a high incidence of anti-apoptotic activity. This combination approach with the advanced statistical methods and the bioinformatical analysis may be useful for finding novel molecular targets for diagnosis and therapy.

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February 2006
Volume 28 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kurokawa Y, Honma K, Takemasa I, Nakamori S, Kita-Matsuo H, Motoori M, Nagano H, Dono K, Ochiya T, Monden M, Monden M, et al: Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma: A new approach to identify novel tumor markers. Int J Oncol 28: 383-391, 2006
APA
Kurokawa, Y., Honma, K., Takemasa, I., Nakamori, S., Kita-Matsuo, H., Motoori, M. ... Kato, K. (2006). Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma: A new approach to identify novel tumor markers. International Journal of Oncology, 28, 383-391. https://doi.org/10.3892/ijo.28.2.383
MLA
Kurokawa, Y., Honma, K., Takemasa, I., Nakamori, S., Kita-Matsuo, H., Motoori, M., Nagano, H., Dono, K., Ochiya, T., Monden, M., Kato, K."Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma: A new approach to identify novel tumor markers". International Journal of Oncology 28.2 (2006): 383-391.
Chicago
Kurokawa, Y., Honma, K., Takemasa, I., Nakamori, S., Kita-Matsuo, H., Motoori, M., Nagano, H., Dono, K., Ochiya, T., Monden, M., Kato, K."Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma: A new approach to identify novel tumor markers". International Journal of Oncology 28, no. 2 (2006): 383-391. https://doi.org/10.3892/ijo.28.2.383