Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel

  • Authors:
    • Ching-Sheng Yeh
    • Jaw-Yuan Wang
    • Chan-Han Wu
    • Inn-Wen Chong
    • Fu-Yen Chung
    • Yung-Hsin Wang
    • Ya-Ping Yu
    • Shiu-Ru Lin
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/ijo.28.2.411
  • Pages: 411-420
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Abstract

The objective of this study was mainly to evaluate the simultaneous detection of expression levels of a multiple mRNA marker panel in the peripheral blood of colorectal cancer (CRC) patients for use in complementary CRC diagnosis. Twenty-seven tumor tissue specimens and 80 peripheral blood specimens were collected from CRC patients. Firstly, the levels of multiple molecular markers in the tumor tissue and blood specimens were evaluated by using real-time quantitative PCR (RT-QPCR) and membrane array. The result of linear regression showed a high degree of correlation (r=0.954, P<0.0001) between the data of these two methods. CK-19 was the marker with the highest detection rate (87.5%) in the peripheral blood, followed by CEA (82.6%), REG4 (80.8%), and then uPA (80.0%) and TLAM1 (80.0%). The levels of the six markers in the peripheral blood were extensively explored. In the 80 patients, the frequency of CK-19, CK-20, CEA, REG4, uPA, and TIAM1 mRNA overexpression was 82.5% (66/80), 78.8% (63/80), 82.5% (66/80), 80.0% (64/80), 78.8% (63/80), and 80.0% (64/80), respectively. Then, a panel combining these 6 mRNA markers was evaluated for its utility in the clinical diagnosis of CRC. The sensitivity, specificity, and accuracy of membrane array-based diagnostic method were 88.8%, 87.8%, and 88.2%, respectively; much higher than those of examinations with single markers. Finally, lymph node metastasis (P=0.024) and TNM stage (P=0.009) were found to be significantly correlated with overexpression of the multiple mRNA marker panel. The detection rates of stage-I and -II CRC by using the multi-marker membrane array were 54.5% (6/11) and 92.0% (23/25), respectively. In conclusion, the results of the present study have shown that this innovative membrane array technique with a multiple mRNA marker panel can significantly improve the diagnosis rate of early colorectal cancer.

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February 2006
Volume 28 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yeh C, Wang J, Wu C, Chong I, Chung F, Wang Y, Yu Y and Lin S: Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel. Int J Oncol 28: 411-420, 2006
APA
Yeh, C., Wang, J., Wu, C., Chong, I., Chung, F., Wang, Y. ... Lin, S. (2006). Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel. International Journal of Oncology, 28, 411-420. https://doi.org/10.3892/ijo.28.2.411
MLA
Yeh, C., Wang, J., Wu, C., Chong, I., Chung, F., Wang, Y., Yu, Y., Lin, S."Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel". International Journal of Oncology 28.2 (2006): 411-420.
Chicago
Yeh, C., Wang, J., Wu, C., Chong, I., Chung, F., Wang, Y., Yu, Y., Lin, S."Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel". International Journal of Oncology 28, no. 2 (2006): 411-420. https://doi.org/10.3892/ijo.28.2.411