Epigallocatechin-3-gallate inhibits the PDGF-induced VEGF expression in human vascular smooth muscle cells via blocking PDGF receptor and Erk-1/2

  • Authors:
    • Jung S. Park
    • Mi H. Kim
    • Hee J. Chang
    • Ki M. Kim
    • Seok M. Kim
    • Boo A. Shin
    • Bong W. Ahn
    • Young D. Jung
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijo.29.5.1247
  • Pages: 1247-1252
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Abstract

Platelet-derived growth factor (PDGF) has been known to induce vascular endothelial growth factor (VEGF) expression in human vascular smooth muscle cells (hVSMCs). We previously reported that Erk-1/2 and AP-1 pathways are crucial in the PDGF-induced VEGF expression in hVSMCs . In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG), the major green tea catechin, on the PDGF-induced VEGF expression in hVSMCs and the underlying mechanisms. EGCG were found to inhibit dose-dependently the VEGF expression and activation of PDGF receptor, Erk-1/2 and AP-1 induced by PDGF. In addition, cell free studies demonstrated that EGCG could directly inhibit the Erk-1/2 activity. Conditioned media from the hVSMCs treated with PDGF could remarkably stimulate the in vitro growth of human umbilical vein endothelial cells (HUVECs) but the media from the EGCG-pretreated hVSMCs lost its stimulatory activity for HUVEC proliferation. These results suggest that EGCG may exert the anti-angiogenic effect by inhibiting the PDGF-induced VEGF expression at multiple signaling levels.

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November 2006
Volume 29 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park JS, Kim MH, Chang HJ, Kim KM, Kim SM, Shin BA, Ahn BW and Jung YD: Epigallocatechin-3-gallate inhibits the PDGF-induced VEGF expression in human vascular smooth muscle cells via blocking PDGF receptor and Erk-1/2. Int J Oncol 29: 1247-1252, 2006
APA
Park, J.S., Kim, M.H., Chang, H.J., Kim, K.M., Kim, S.M., Shin, B.A. ... Jung, Y.D. (2006). Epigallocatechin-3-gallate inhibits the PDGF-induced VEGF expression in human vascular smooth muscle cells via blocking PDGF receptor and Erk-1/2. International Journal of Oncology, 29, 1247-1252. https://doi.org/10.3892/ijo.29.5.1247
MLA
Park, J. S., Kim, M. H., Chang, H. J., Kim, K. M., Kim, S. M., Shin, B. A., Ahn, B. W., Jung, Y. D."Epigallocatechin-3-gallate inhibits the PDGF-induced VEGF expression in human vascular smooth muscle cells via blocking PDGF receptor and Erk-1/2". International Journal of Oncology 29.5 (2006): 1247-1252.
Chicago
Park, J. S., Kim, M. H., Chang, H. J., Kim, K. M., Kim, S. M., Shin, B. A., Ahn, B. W., Jung, Y. D."Epigallocatechin-3-gallate inhibits the PDGF-induced VEGF expression in human vascular smooth muscle cells via blocking PDGF receptor and Erk-1/2". International Journal of Oncology 29, no. 5 (2006): 1247-1252. https://doi.org/10.3892/ijo.29.5.1247