CYTOGENETIC STUDIES ON THE IN-VITRO GENOTOXICITY OF 4-NITROQUINOLINE-1-OXIDE ON HUMAN-LYMPHOCYTES

  • Authors:
    • TC HSU
    • N SAVARAJI
    • Z TRIZNA
    • L FEUN
    • C FURLONG
    • SP SCHANTZ
    • L SHIRLEY
  • View Affiliations

  • Published online on: November 1, 1993     https://doi.org/10.3892/ijo.3.5.823
  • Pages: 823-826
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Abstract

Using the UV-mimetic mutagen 4-nitroquinoline-1-oxide (4NQO) to induce genetic damage in human cells (lymphoblastoid lines and primary cultures of peripheral blood samples), chromosome aberrations were induced by treating the cells with 4NQO at 1 X 10(-5) M for 24 h. The overwhelming majority of chromosome aberrations was of the chromatid (S + G2) type instead of the chromosome (G1) type. The most common chromatid aberrations were simple breaks, isochromatid breaks, and chromatid exchanges. When the number of chromatid breaks per cell value was used as a measurement for 4NQO sensitivity, lymphoblastoid cells from a xeroderma pigmentosum patient showed the highest sensitivity, followed by the cells of two melanoma patients and normal persons. These preliminary results suggest that 4NQO may be employed to develop an assay system as a biomarker for determining UV sensitivity in the human population.

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November 1993
Volume 3 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
HSU T, SAVARAJI N, TRIZNA Z, FEUN L, FURLONG C, SCHANTZ S and SHIRLEY L: CYTOGENETIC STUDIES ON THE IN-VITRO GENOTOXICITY OF 4-NITROQUINOLINE-1-OXIDE ON HUMAN-LYMPHOCYTES. Int J Oncol 3: 823-826, 1993
APA
HSU, T., SAVARAJI, N., TRIZNA, Z., FEUN, L., FURLONG, C., SCHANTZ, S., & SHIRLEY, L. (1993). CYTOGENETIC STUDIES ON THE IN-VITRO GENOTOXICITY OF 4-NITROQUINOLINE-1-OXIDE ON HUMAN-LYMPHOCYTES. International Journal of Oncology, 3, 823-826. https://doi.org/10.3892/ijo.3.5.823
MLA
HSU, T., SAVARAJI, N., TRIZNA, Z., FEUN, L., FURLONG, C., SCHANTZ, S., SHIRLEY, L."CYTOGENETIC STUDIES ON THE IN-VITRO GENOTOXICITY OF 4-NITROQUINOLINE-1-OXIDE ON HUMAN-LYMPHOCYTES". International Journal of Oncology 3.5 (1993): 823-826.
Chicago
HSU, T., SAVARAJI, N., TRIZNA, Z., FEUN, L., FURLONG, C., SCHANTZ, S., SHIRLEY, L."CYTOGENETIC STUDIES ON THE IN-VITRO GENOTOXICITY OF 4-NITROQUINOLINE-1-OXIDE ON HUMAN-LYMPHOCYTES". International Journal of Oncology 3, no. 5 (1993): 823-826. https://doi.org/10.3892/ijo.3.5.823