INVASIVE NEOPLASTIC UROEPITHELIAL CELLS EXPRESS HIGH-LEVELS OF UROKINASE RECEPTOR AND PLASMINOGEN RECEPTOR, ALPHA-ENOLASE

  • Authors:
    • K KARIKO
    • SB MALKOWICZ
    • WJ LI
    • A KUO
    • ES BARNATHAN
  • View Affiliations

  • Published online on: December 1, 1993     https://doi.org/10.3892/ijo.3.6.1089
  • Pages: 1089-1095
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Abstract

Urokinase plasminogen activator (uPA) has been proposed to play a key role in metastatic process of a variety of carcinoma by promoting plasmin mediated tissue degradation. Metastatic cell invasion requires localized proteolysis which could be directed by uPA receptor (uPAR) and a putative plasminogen receptor, alpha-enolase. We have determined invasive potentials of established human uroepithelial cell lines in in vitro assays. The cells were found to be highly invasive (T24, J82, 5637) or slightly invasive (TCCSUP, HT-1376, RT4). The cells were further studied to determine uPAR display on the cell surface, and mRNA expression for uPA, uPAR, alpha-enolase and plasminogen activator inhibitor type-1 (PAI-1). Weakly invasive cells demonstrated a very low quantity of uPAR or alpha-enolase-related products, or elevated level of PAI-1 mRNA. The highly invasive phenotype, however, was associated with an increased level of both uPAR production and alpha-enolase expression. Pretreatment of the highly invasive cells with tranexamic acid, that inhibits binding of both plasminogen and plasmin to the cells, resulted in a significant reduction in cellular invasiveness. These results suggest that uPAR and putative plasminogen receptor alpha-enolase are contributors to cellular invasiveness of neoplastic human uroepithelial cells.

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December 1993
Volume 3 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
KARIKO K, MALKOWICZ S, LI W, KUO A and BARNATHAN E: INVASIVE NEOPLASTIC UROEPITHELIAL CELLS EXPRESS HIGH-LEVELS OF UROKINASE RECEPTOR AND PLASMINOGEN RECEPTOR, ALPHA-ENOLASE. Int J Oncol 3: 1089-1095, 1993
APA
KARIKO, K., MALKOWICZ, S., LI, W., KUO, A., & BARNATHAN, E. (1993). INVASIVE NEOPLASTIC UROEPITHELIAL CELLS EXPRESS HIGH-LEVELS OF UROKINASE RECEPTOR AND PLASMINOGEN RECEPTOR, ALPHA-ENOLASE. International Journal of Oncology, 3, 1089-1095. https://doi.org/10.3892/ijo.3.6.1089
MLA
KARIKO, K., MALKOWICZ, S., LI, W., KUO, A., BARNATHAN, E."INVASIVE NEOPLASTIC UROEPITHELIAL CELLS EXPRESS HIGH-LEVELS OF UROKINASE RECEPTOR AND PLASMINOGEN RECEPTOR, ALPHA-ENOLASE". International Journal of Oncology 3.6 (1993): 1089-1095.
Chicago
KARIKO, K., MALKOWICZ, S., LI, W., KUO, A., BARNATHAN, E."INVASIVE NEOPLASTIC UROEPITHELIAL CELLS EXPRESS HIGH-LEVELS OF UROKINASE RECEPTOR AND PLASMINOGEN RECEPTOR, ALPHA-ENOLASE". International Journal of Oncology 3, no. 6 (1993): 1089-1095. https://doi.org/10.3892/ijo.3.6.1089