Transfer of in vitro expanded T lymphocytes after activation with dendritomas prolonged survival of mice challenged with EL4 tumor cells

  • Authors:
    • Jinhua Li
    • Leigh Theofanous
    • Sara Stickel
    • Hilary Bouton-Verville
    • Kelly E. Burgin
    • Susan Jakubchak
    • Thomas E. Wagner
    • Yanzhang Wei
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/ijo.31.1.193
  • Pages: 193-197
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Abstract

Adoptive T cell transfer after in vitro expansion represents an attractive cancer immunotherapy. The majority of studies so far have been focusing on the expansion of tumor infiltrated lymphocytes (TIL) and some have shown very encouraging results. Recently, we have developed a unique tumor immune response activator, dendritomas, by fusion of dendritic cells and tumor cells. Animal studies and early clinical trials have shown that dendritomas are able to activate tumor specific immune responses. In this study, we hypothesized that naïve T cells can be primed with dendritomas and expanded in vitro to develop an adoptive transfer therapy for patients who do not have solid tumors, such as leukemia. T cells were isolated and purified from lymph nodes of mice. The cells were then incubated with dendritomas made from syngeneic DCs and tumor cells and expanded in vitro using Dynabeads mouse CD3/CD28 T cell expander for approximately three weeks. The in vitro primed and expanded T cells showed tumor cell specific CTL activity and increased secretion of IFN-γ. Tumor bearing mice receiving the in vitro expanded T cells survived significantly longer than control mice. Furthermore, the depletion of regulator T cells enhanced the survival of the mice that received the adoptive transfer therapy.

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July 2007
Volume 31 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li J, Theofanous L, Stickel S, Bouton-Verville H, Burgin KE, Jakubchak S, Wagner TE and Wei Y: Transfer of in vitro expanded T lymphocytes after activation with dendritomas prolonged survival of mice challenged with EL4 tumor cells. Int J Oncol 31: 193-197, 2007
APA
Li, J., Theofanous, L., Stickel, S., Bouton-Verville, H., Burgin, K.E., Jakubchak, S. ... Wei, Y. (2007). Transfer of in vitro expanded T lymphocytes after activation with dendritomas prolonged survival of mice challenged with EL4 tumor cells. International Journal of Oncology, 31, 193-197. https://doi.org/10.3892/ijo.31.1.193
MLA
Li, J., Theofanous, L., Stickel, S., Bouton-Verville, H., Burgin, K. E., Jakubchak, S., Wagner, T. E., Wei, Y."Transfer of in vitro expanded T lymphocytes after activation with dendritomas prolonged survival of mice challenged with EL4 tumor cells". International Journal of Oncology 31.1 (2007): 193-197.
Chicago
Li, J., Theofanous, L., Stickel, S., Bouton-Verville, H., Burgin, K. E., Jakubchak, S., Wagner, T. E., Wei, Y."Transfer of in vitro expanded T lymphocytes after activation with dendritomas prolonged survival of mice challenged with EL4 tumor cells". International Journal of Oncology 31, no. 1 (2007): 193-197. https://doi.org/10.3892/ijo.31.1.193