Purified salograviolide a isolated from centaurea ainetensis causes growth inhibition and apoptosis in neoplastic epidermal cells

  • Authors:
    • Akram Ghantous
    • Ahmad Abou Tayyoun
    • Ghada Abou Lteif
    • Najat A. Saliba
    • Hala Gali-Muhtasib
    • Marwan El-Sabban
    • Nadine Darwiche
  • View Affiliations

  • Published online on: April 1, 2008     https://doi.org/10.3892/ijo.32.4.841
  • Pages: 841-849
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Abstract

Many of the best-selling anticancer drugs are plant-derived. We tested for the anticancer properties of extracts isolated from Centaurea ainetensis, a plant species endemic to Lebanon and which is often used in folk medicine. We performed bioassay-guided fractionation of Centaurea ainetensis extracts using a panel of normal and neoplastic murine cells to identify a component that is associated with antitumor activities. Among several compounds that were fractionated, the sesquiterpene lactone, Salograviolide A, was identified and found to exert the most significant growth inhibitory effects on neoplastic cells. At concentrations that were non-cytotoxic to primary keratinocytes, Centaurea ainetensis crude extract and Salograviolide A preferentially inhibited the proliferation of papilloma and squamous cell carcinoma (SCC) cell lines without significantly affecting the growth of normal cells. Flow cytometric analysis of DNA content indicated that the inhibition of cell proliferation by Centaurea ainetensis crude extract and Salograviolide A was due to G0/G1 cell cycle arrest and increased pre-G0/G1, respectively. The increase in pre-G0/G1, and presumably apoptosis induction, in Salograviolide A-treated keratinocytes was confirmed by DNA Hoechst staining. Western blot analysis and electrophoretic mobility shift assay showed that both the crude extract and the isolated molecule differentially modulated key cell cycle and apoptotic regulators as well as NF-κB signaling. Salograviolide A-induced growth inhibition in neoplastic cells was mediated by the accumulation of reactive oxygen species (ROS) highlighting a potent oxidant role of this molecule. These studies suggest the potential therapeutic effects of Centaurea ainetensis, and its component, Salograviolide A, against epidermal squamous cell carcinogenesis.

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April 2008
Volume 32 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ghantous A, Tayyoun AA, Lteif GA, Saliba NA, Gali-Muhtasib H, El-Sabban M and Darwiche N: Purified salograviolide a isolated from centaurea ainetensis causes growth inhibition and apoptosis in neoplastic epidermal cells. Int J Oncol 32: 841-849, 2008
APA
Ghantous, A., Tayyoun, A.A., Lteif, G.A., Saliba, N.A., Gali-Muhtasib, H., El-Sabban, M., & Darwiche, N. (2008). Purified salograviolide a isolated from centaurea ainetensis causes growth inhibition and apoptosis in neoplastic epidermal cells. International Journal of Oncology, 32, 841-849. https://doi.org/10.3892/ijo.32.4.841
MLA
Ghantous, A., Tayyoun, A. A., Lteif, G. A., Saliba, N. A., Gali-Muhtasib, H., El-Sabban, M., Darwiche, N."Purified salograviolide a isolated from centaurea ainetensis causes growth inhibition and apoptosis in neoplastic epidermal cells". International Journal of Oncology 32.4 (2008): 841-849.
Chicago
Ghantous, A., Tayyoun, A. A., Lteif, G. A., Saliba, N. A., Gali-Muhtasib, H., El-Sabban, M., Darwiche, N."Purified salograviolide a isolated from centaurea ainetensis causes growth inhibition and apoptosis in neoplastic epidermal cells". International Journal of Oncology 32, no. 4 (2008): 841-849. https://doi.org/10.3892/ijo.32.4.841