Theranostic proteomic profiling of cyclins, cyclin dependent kinases and Ras in human cancer cell lines is dependent on p53 mutational status

  • Authors:
    • Hilmar M. Warenius
    • Laurence Seabra
    • Lito Kyritsi
    • Ros White
    • Roisin Dormer
    • Shanez Anandappa
    • Carole Thomas
    • Amanda Howarth
  • View Affiliations

  • Published online on: April 1, 2008     https://doi.org/10.3892/ijo.32.4.895
  • Pages: 895-907
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Despite major advances in the molecular biology of the cancer cell over the past two decades, the great majority of patients are still treated by conventional cytotoxic drugs. The chemotherapy regimens employed frequently include platinating agents, taxanes, intercalating agents and topoisomerase inhibitors. Attempts to predict the therapeutic efficacy of such drugs by molecular profiling (theranostics) have up to the present time had limited success. Genes responsible for the control of cell division, senescence and apoptosis whose normal functions become corrupted during carcinogenesis, might potentially play a part in determining chemotherapeutic response. Here we have examined the relationships between the chemoresponsiveness of 18 human in vitro cancer cell lines and proteomic expression of Ras, cyclins B1 and D1 and cyclin-dependent kinases Cdk1 and Cdk4. When all 18 cell lines were examined as a single group, proteomic expression did not provide any helpful theranostic predictors. Clear relationships between proteomic expression and drug efficacy emerged, however, when Ras, cyclin B1, cyclin D1, Cdk1 and Cdk4 were examined separately in p53 wild-type and p53 mutant cell subsets. We suggest that the theranostic relationships we have detected in vitro may have potential relevance in vivo and should prompt clinical theranostic studies which take account of p53 mutational status.

Related Articles

Journal Cover

April 2008
Volume 32 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Warenius HM, Seabra L, Kyritsi L, White R, Dormer R, Anandappa S, Thomas C and Howarth A: Theranostic proteomic profiling of cyclins, cyclin dependent kinases and Ras in human cancer cell lines is dependent on p53 mutational status. Int J Oncol 32: 895-907, 2008
APA
Warenius, H.M., Seabra, L., Kyritsi, L., White, R., Dormer, R., Anandappa, S. ... Howarth, A. (2008). Theranostic proteomic profiling of cyclins, cyclin dependent kinases and Ras in human cancer cell lines is dependent on p53 mutational status. International Journal of Oncology, 32, 895-907. https://doi.org/10.3892/ijo.32.4.895
MLA
Warenius, H. M., Seabra, L., Kyritsi, L., White, R., Dormer, R., Anandappa, S., Thomas, C., Howarth, A."Theranostic proteomic profiling of cyclins, cyclin dependent kinases and Ras in human cancer cell lines is dependent on p53 mutational status". International Journal of Oncology 32.4 (2008): 895-907.
Chicago
Warenius, H. M., Seabra, L., Kyritsi, L., White, R., Dormer, R., Anandappa, S., Thomas, C., Howarth, A."Theranostic proteomic profiling of cyclins, cyclin dependent kinases and Ras in human cancer cell lines is dependent on p53 mutational status". International Journal of Oncology 32, no. 4 (2008): 895-907. https://doi.org/10.3892/ijo.32.4.895