Open Access

Involvement of RQCD1 overexpression, a novel cancer-testis antigen, in the Akt pathway in breast cancer cells

  • Authors:
    • Masahiko Ajiro
    • Toyomasa Katagiri
    • Koji Ueda
    • Hidewaki Nakagawa
    • Chikako Fukukawa
    • Meng-Lay Lin
    • Jae-Hyun Park
    • Toshihiko Nishidate
    • Yataro Daigo
    • Yusuke Nakamura
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijo_00000379
  • Pages: 673-681
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We here report identification and characterization of Required for Cell Differentiation 1 homolog (RQCD1) as a potential therapeutic target for breast cancer. Gene-expression profiling analysis of breast cancer cells, semi-quantitative RT-PCR, Northern blotting and Western blotting confirmed RQCD1 to be frequently up-regulated in breast cancer specimens and breast cancer cell lines. On the other hand, its expression was very weak or hardly detectable in normal human tissues except testis, indicating this molecule to be a novel cancer-testis antigen. Treatment of breast cancer cell lines with siRNA targeting RQCD1 drastically suppressed cell proliferation. Concordantly, introduction of exogenous RQCD1 into HEK293 cells significantly enhanced cell growth, implying RQCD1 to have an oncogenic activity. Co-immunoprecipitation experiments and immunocytochemical staining revealed an interaction of RQCD1 protein with Grb10 interacting GYF protein 1 (GIGYF1) and 2 (GIGYF2) proteins, involved in regulation of Akt activation, in breast cancer cells. Interestingly, knockdown of either of RQCD1, GIGYF1 or GIGYF2 resulted in significant reduction of the phosphorylation of Akt at Ser 473 in breast cancer cell lines. Our findings suggest that RQCD1 is a potential molecular target for treatment of breast cancer.

Related Articles

Journal Cover

October 2009
Volume 35 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ajiro M, Katagiri T, Ueda K, Nakagawa H, Fukukawa C, Lin M, Park J, Nishidate T, Daigo Y, Nakamura Y, Nakamura Y, et al: Involvement of RQCD1 overexpression, a novel cancer-testis antigen, in the Akt pathway in breast cancer cells. Int J Oncol 35: 673-681, 2009
APA
Ajiro, M., Katagiri, T., Ueda, K., Nakagawa, H., Fukukawa, C., Lin, M. ... Nakamura, Y. (2009). Involvement of RQCD1 overexpression, a novel cancer-testis antigen, in the Akt pathway in breast cancer cells. International Journal of Oncology, 35, 673-681. https://doi.org/10.3892/ijo_00000379
MLA
Ajiro, M., Katagiri, T., Ueda, K., Nakagawa, H., Fukukawa, C., Lin, M., Park, J., Nishidate, T., Daigo, Y., Nakamura, Y."Involvement of RQCD1 overexpression, a novel cancer-testis antigen, in the Akt pathway in breast cancer cells". International Journal of Oncology 35.4 (2009): 673-681.
Chicago
Ajiro, M., Katagiri, T., Ueda, K., Nakagawa, H., Fukukawa, C., Lin, M., Park, J., Nishidate, T., Daigo, Y., Nakamura, Y."Involvement of RQCD1 overexpression, a novel cancer-testis antigen, in the Akt pathway in breast cancer cells". International Journal of Oncology 35, no. 4 (2009): 673-681. https://doi.org/10.3892/ijo_00000379