Role of the AKT pathway in microRNA expression of human U251 glioblastoma cells

Retraction in: /10.3892/ijo.2023.5597

  • Authors:
    • Xuan Zhou
    • Yu Ren
    • Lei Han
    • Mei Mei
    • Peng Xu
    • Chun-Zhi Zhang
    • Guang-Xiu Wang
    • Zhi-Fan Jia
    • Pei-Yu Pu
    • Chun-Sheng Kang
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/ijo_00000542
  • Pages: 665-672
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Abstract

Activation of the AKT (serine-threonine kinase) pathway is a common feature in glioblastoma cells. Downstream factors of the AKT pathway are involved in cell proliferation, apoptosis, cellular migration and angiogenesis. Micro-RNAs (miRNAs) are highly conserved small non-coding RNAs that block targeted mRNA expression at the post-transcriptional level. The aim of this study was to investigate the role of the AKT pathway in regulating miRNA. The changes of miRNA expression profile in human glioblastome U251 cells after AKT small interfering RNA transfection were examined by a microarray, and confirmed by Northern blotting. Down-regulation of AKT expression by siRNA decreased the activity of AKT pathway in U251 cells. Interruption of AKT pathway suppressed the expression of NF-κB and c-Myc, furthermore, the expression of a set of miRNAs was also changed after AKT siRNA transfection. There are putative binding sites of NF-κB and c-Myc in the promoters of several up-regulated miRNAs, indicating these transcription factors may also be involved in the regulation of miRNA expression, thus affecting the activity of AKT pathway in tumorigenesis. We provide new components of the regulatory function of AKT pathway to better understand the regulatory network mediated by downstream transcription factors. The understanding of the regulatory function of AKT pathway is crucial in tailored therapy of gliomas.

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March 2010
Volume 36 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhou X, Ren Y, Han L, Mei M, Xu P, Zhang C, Wang G, Jia Z, Pu P, Kang C, Kang C, et al: Role of the AKT pathway in microRNA expression of human U251 glioblastoma cells Retraction in /10.3892/ijo.2023.5597. Int J Oncol 36: 665-672, 2010
APA
Zhou, X., Ren, Y., Han, L., Mei, M., Xu, P., Zhang, C. ... Kang, C. (2010). Role of the AKT pathway in microRNA expression of human U251 glioblastoma cells Retraction in /10.3892/ijo.2023.5597. International Journal of Oncology, 36, 665-672. https://doi.org/10.3892/ijo_00000542
MLA
Zhou, X., Ren, Y., Han, L., Mei, M., Xu, P., Zhang, C., Wang, G., Jia, Z., Pu, P., Kang, C."Role of the AKT pathway in microRNA expression of human U251 glioblastoma cells Retraction in /10.3892/ijo.2023.5597". International Journal of Oncology 36.3 (2010): 665-672.
Chicago
Zhou, X., Ren, Y., Han, L., Mei, M., Xu, P., Zhang, C., Wang, G., Jia, Z., Pu, P., Kang, C."Role of the AKT pathway in microRNA expression of human U251 glioblastoma cells Retraction in /10.3892/ijo.2023.5597". International Journal of Oncology 36, no. 3 (2010): 665-672. https://doi.org/10.3892/ijo_00000542