Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma

  • Authors:
    • Babett Bartling
    • Grit Rehbein
    • Andreas Simm
    • Rolf-Edgar Silber
    • Hans-Stefan Hofmann
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/ijo_00000582
  • Pages: 1015-1021
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The ο-acyltransferase Porcupine contributes to secretion and function of Wnt signaling molecules, which stimulate the expression of various cancer-related genes. Porcupine is also involved in the Wnt-induced cell signaling via β-Catenin in non-small cell lung carcinoma (NSCLC) cells. Herein, we report that the expression level of Porcupine in human NSCLC tissues (n=89) positively correlates with the expression of several genes coding for cancer-related molecules such as β-Catenin, Hypoxia-inducible factor-1α and Jun B. However, the mRNA expression of Porcupine was not generally increased in NSCLC compared to normal lung tissues. In NSCLC tissues we also found a positive correlation between the expression level of Porcupine and the calcium-binding protein S100P, which contributes to initiation and invasion of cancer cells. Subsequent studies showed that the DNA hypomethylation with 5-aza-2'deoxycytidine increased the mRNA expression of S100P in NSCLC cells but did not alter that of Porcupine. Silencing the expression of Porcupine with small interfering (si)RNA reduced the expression of S100P in NSCLC cells, whereas silencing the expression of S100P with siRNA did not effect the level of Porcupine expression. In conclusion, besides DNA methylation processes, Porcupine might regulate the expression of some cancer-related molecules including S100P in human NSCLC.

Related Articles

Journal Cover

April 2010
Volume 36 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bartling B, Rehbein G, Simm A, Silber R and Hofmann H: Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma. Int J Oncol 36: 1015-1021, 2010
APA
Bartling, B., Rehbein, G., Simm, A., Silber, R., & Hofmann, H. (2010). Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma. International Journal of Oncology, 36, 1015-1021. https://doi.org/10.3892/ijo_00000582
MLA
Bartling, B., Rehbein, G., Simm, A., Silber, R., Hofmann, H."Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma". International Journal of Oncology 36.4 (2010): 1015-1021.
Chicago
Bartling, B., Rehbein, G., Simm, A., Silber, R., Hofmann, H."Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma". International Journal of Oncology 36, no. 4 (2010): 1015-1021. https://doi.org/10.3892/ijo_00000582