Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo

  • Authors:
    • Satoshi Takenaka
    • Norifumi Naka
    • Nobuhito Araki
    • Nobuyuki Hashimoto
    • Takafumi Ueda
    • Kiyoko Yoshioka
    • Hideki Yoshikawa
    • Kazuyuki Itoh
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/ijo_00000559
  • Pages: 823-831
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Abstract

Synovial sarcoma (SS) is an aggressive soft-tissue malignancy characterized by a unique t(X;18) translocation resulting in expression of SS18-SSX fusion protein. In order to investigate the biological function of this fusion protein and to develop a novel therapeutic option, we examined downregulation of SS18-SSX1 expression by small interfering RNA targeting SS18-SSX1 in three human SS cell lines. Microarray analysis comparing SS18-SSX1-silenced cells with control cells in three SS cell lines showed that SS18-SSX1 mainly affected the focal adhesion pathway. In accord with the array data, silencing of SS18-SSX1 enhances adhesion to the extracellular matrix through the induction of expression of myosin light-chain kinase. Furthermore, the silencing of SS18-SSX1 inhibits anchorage-independent growth in vitro and systemic delivery of siRNA against SS18-SSX1 using a nanoparticle system inhibited tumor growth in a nude mouse xenograft model. Our results demonstrate that siRNA targeting of SS18-SSX1 has therapeutic potential for the treatment of SS.

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April 2010
Volume 36 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Takenaka S, Naka N, Araki N, Hashimoto N, Ueda T, Yoshioka K, Yoshikawa H and Itoh K: Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. Int J Oncol 36: 823-831, 2010
APA
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K. ... Itoh, K. (2010). Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo. International Journal of Oncology, 36, 823-831. https://doi.org/10.3892/ijo_00000559
MLA
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K., Yoshikawa, H., Itoh, K."Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo". International Journal of Oncology 36.4 (2010): 823-831.
Chicago
Takenaka, S., Naka, N., Araki, N., Hashimoto, N., Ueda, T., Yoshioka, K., Yoshikawa, H., Itoh, K."Downregulation of SS18-SSX1 expression in synovial sarcoma by small interfering RNA enhances the focal adhesion pathway and inhibits anchorage-independent growth in vitro and tumor growth in vivo". International Journal of Oncology 36, no. 4 (2010): 823-831. https://doi.org/10.3892/ijo_00000559