The immunoglobulin-like cell adhesion molecule hepaCAM is cleaved in the human breast carcinoma MCF7 cells

  • Authors:
    • Ting Zhang
    • Mei C. Moh
    • Lay H. Lee
    • Shali Shen
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijo_00000663
  • Pages: 155-165
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Abstract

We previously reported the identification and characteristics of hepaCAM, a new immunoglobulin-like adhesion molecule. Frequently lost in diverse tumors, hepaCAM exhibits antiproliferative effects on cancer cells and promotes cell-extracellular matrix (ECM) interactions when re-expressed. Herein, we demonstrate for the first time that hepaCAM is cleaved in the human breast carcinoma MCF7 cells, generating a fragment containing mainly the cytoplasmic domain. The phorbol ester phorbol 12-myristate 13-acetate (PMA) did not affect the cleavage of hepaCAM. However, calcium-influx promoted hepaCAM cleavage independent of PKC. In addition, inhibitors of proteasome and cysteine proteases strongly suppressed the cleavage of hepaCAM, indicating the involvement of proteasome, calpain-1 and cathepsin B. Furthermore, we showed that functions of hepaCAM were impaired when the cytoplasmic domain was cleaved. The truncation mutant of hepaCAM failed to promote cell-ECM adhesion and migration, and lost the inhibitory effects on cell growth, suggesting a regulatory role of the cleavage in hepaCAM functions.

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July 2010
Volume 37 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zhang, T., Moh, M.C., Lee, L.H., & Shen, S. (2010). The immunoglobulin-like cell adhesion molecule hepaCAM is cleaved in the human breast carcinoma MCF7 cells. International Journal of Oncology, 37, 155-165. https://doi.org/10.3892/ijo_00000663
MLA
Zhang, T., Moh, M. C., Lee, L. H., Shen, S."The immunoglobulin-like cell adhesion molecule hepaCAM is cleaved in the human breast carcinoma MCF7 cells". International Journal of Oncology 37.1 (2010): 155-165.
Chicago
Zhang, T., Moh, M. C., Lee, L. H., Shen, S."The immunoglobulin-like cell adhesion molecule hepaCAM is cleaved in the human breast carcinoma MCF7 cells". International Journal of Oncology 37, no. 1 (2010): 155-165. https://doi.org/10.3892/ijo_00000663