Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin

  • Authors:
    • Lucia Cicchillitti
    • Anna Della Corte
    • Michela Di Michele
    • Maria Benedetta Donati
    • Domenico Rotilio
    • Giovanni Scambia
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/ijo_00000693
  • Pages: 445-454
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Abstract

Ovarian cancer is the second most frequently diagnosed malignancy of the reproductive system and is the leading cause of gynecological cancer mortality. Although the majority of advanced ovarian carcinomas initially respond successfully to taxane-based chemotherapy, resistance to chemotherapy remains the primary factor accounting for the low 5-year survival in this patient population. Recent data obtained by our group demonstrate that the disulphide isomerase ERp57 is strongly modulated in paclitaxel resistance suggesting that it may represent a chemoresistance biomarker in ovarian cancer. In the present study, we characterise a nuclear multimeric complex where ERp57 is associated with protein species involved in cell division and gene expression, as Nucleolin, Nucleophosmin, Vimentin, Aurora kinase C and β-actin. In particular, we show that the occurrence of the interaction of nuclear ERp57 with β-actin is associated with paclitaxel resistance and that specific actin conformations modulate this complex. We propose the involvement of the nuclear ERp57 complex in mechanisms associated with chromosome segregation in which specific conformational states of actin play a role in the pathway involved in paclitaxel resistance.

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August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Cicchillitti L, Della Corte A, Di Michele M, Donati MB, Rotilio D and Scambia G: Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin. Int J Oncol 37: 445-454, 2010
APA
Cicchillitti, L., Della Corte, A., Di Michele, M., Donati, M.B., Rotilio, D., & Scambia, G. (2010). Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin. International Journal of Oncology, 37, 445-454. https://doi.org/10.3892/ijo_00000693
MLA
Cicchillitti, L., Della Corte, A., Di Michele, M., Donati, M. B., Rotilio, D., Scambia, G."Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin". International Journal of Oncology 37.2 (2010): 445-454.
Chicago
Cicchillitti, L., Della Corte, A., Di Michele, M., Donati, M. B., Rotilio, D., Scambia, G."Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin". International Journal of Oncology 37, no. 2 (2010): 445-454. https://doi.org/10.3892/ijo_00000693