TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells

  • Authors:
    • Yen Ta Huang
    • Pei Chun Lai
    • Chia Chen Wu
    • Chuan Chu Cheng
    • Ted H. Chiu
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/ijo_00000745
  • Pages: 943-949
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Abstract

BDNF (brain-derived neurotrophic factor) and its receptor TrkB (tropomyosin receptor kinase B) play important roles in the progression of cancer, including transitional cell carcinoma (TCC) cells reported in our previous investigation. In this study, we used a specific TrkB antibody (Ab) to evaluate its effects on survival, proliferation and migration/invasion in three TCC cell lines (BFTC905, T24 and TSGH8301) in vitro. The TrkB Ab at 1 and 3 µg/ml, but not the TrkA or TrkC Abs, significantly elicited cytotoxicity in TCC cells. The TrkB Ab at 3 µg/ml also induced apoptosis of TCC cells, which may result from up-regulation of phospho-p38 plus down-regulation of survivin and securin expression. The TrkB Ab at 0.5 µg/ml, which did not show cytotoxicity, suppressed migration of TCC cells and invasion of BFTC905 cells, possibly mediated through increased E-cadherin, decreased BDNF-stimulated phospho-PLCγ1 and reduced MMP-9 activity. These results indicate that TrkB blockade may be a new strategy for TCC therapy.

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October 2010
Volume 37 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Huang YT, Lai PC, Wu CC, Cheng CC and Chiu TH: TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells. Int J Oncol 37: 943-949, 2010
APA
Huang, Y.T., Lai, P.C., Wu, C.C., Cheng, C.C., & Chiu, T.H. (2010). TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells. International Journal of Oncology, 37, 943-949. https://doi.org/10.3892/ijo_00000745
MLA
Huang, Y. T., Lai, P. C., Wu, C. C., Cheng, C. C., Chiu, T. H."TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells". International Journal of Oncology 37.4 (2010): 943-949.
Chicago
Huang, Y. T., Lai, P. C., Wu, C. C., Cheng, C. C., Chiu, T. H."TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells". International Journal of Oncology 37, no. 4 (2010): 943-949. https://doi.org/10.3892/ijo_00000745