Regulation of cancer cell proliferation by caveolin-2 down-regulation and re-expression

  • Authors:
    • Sangho Lee
    • Hayeong Kwon
    • Kyuho Jeong
    • Yunbae Pak
  • View Affiliations

  • Published online on: March 3, 2011     https://doi.org/10.3892/ijo.2011.958
  • Pages: 1395-1402
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Abstract

We investigated whether altering caveolin-2 (cav-2) expression affects the proliferation of cancer cells. Cav-2 was not detected in HepG2, SH-SY5Y and LN-CaP cells, and the loss of cav-2 expression was not restored by 5-aza-2'-deoxycytidine treatment. In contrast, C6, HeLa, A549, MCF7 and PC3M cells expressed cav-2. Effects of re-expression of exogenous cav-2 in HepG2, SH-SY5Y and LN-CaP cells, and siRNA-mediated down-regulation of endogenous cav-2 in C6, HeLa, A549, MCF7 and PC3M cells on cancer proliferation were examined by MTT assay, colony formation assay and flow cytometric analysis. Cav-2 transfection in HepG2 hepatocellular carcinoma cells and knockdown in C6 glioma cells caused reduction in cell proliferation and growth with retarded entry into the S phase. Cav-2 re-expression in SH-SY5Y neuroblastoma cells and depletion in HeLa epithelial cervical cancer and A549 lung adenocarcinoma cells promoted cancer cell proliferation. Luciferase reporter assay showed that transcriptional activation of Elk-1 and STAT3 was significantly decreased in cav-2-transfected HepG2 hepatocellular carcinoma and down-regulated C6 glioma cells. Our data suggest that cav-2 acts as a modulator of cancer progression.

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May 2011
Volume 38 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lee S, Kwon H, Jeong K and Pak Y: Regulation of cancer cell proliferation by caveolin-2 down-regulation and re-expression. Int J Oncol 38: 1395-1402, 2011
APA
Lee, S., Kwon, H., Jeong, K., & Pak, Y. (2011). Regulation of cancer cell proliferation by caveolin-2 down-regulation and re-expression. International Journal of Oncology, 38, 1395-1402. https://doi.org/10.3892/ijo.2011.958
MLA
Lee, S., Kwon, H., Jeong, K., Pak, Y."Regulation of cancer cell proliferation by caveolin-2 down-regulation and re-expression". International Journal of Oncology 38.5 (2011): 1395-1402.
Chicago
Lee, S., Kwon, H., Jeong, K., Pak, Y."Regulation of cancer cell proliferation by caveolin-2 down-regulation and re-expression". International Journal of Oncology 38, no. 5 (2011): 1395-1402. https://doi.org/10.3892/ijo.2011.958