Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner

  • Authors:
    • Sayuri Kawamoto
    • Kenta Moriwaki
    • Tsutomu Nakagawa
    • Mika Terao
    • Shinichiro Shinzaki
    • Naoko Yamane-Ohnuki
    • Mitsuo Satoh
    • Anand S. Mehta
    • Timothy M. Block
    • Eiji Miyoshi
  • View Affiliations

  • Published online on: April 14, 2011     https://doi.org/10.3892/ijo.2011.1005
  • Pages: 203-208
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Golgi phosphoprotein 2 (GP73) is a type II Golgi protein, which was found on examination of the fucosylated proteome as a potential tumor marker for hepatocellular carcinoma (HCC). The serum levels of both total and fucosylated GP73 were increased in the sera of patients with HCC. Fucosylation is one of the most important oligosaccharide modifications involved in cancer and is catalyzed by α1,6-fucosyltransferase (Fut8). In the present study, we investigated the effect of Fut8 overexpression on GP73 production in the human hepatoma cell line Hep3B. The Fut8 expression vector was transfected into Hep3B cells and the expression of GP73 was investigated by Western blotting and real-time PCR. Overexpression of Fut8 dramatically enhanced the expression of GP73 at the transcriptional level. Surprisingly, this effect was not dependent on cellular fucosylation. Overexpression of a mutant Fut8, which was unable to be localized to the Golgi, did not induce GP73 production, suggesting that the localization of Fut8 in the Golgi apparatus was important for the increase in GP73 expression. This is the first demonstration of GP73 regulation through overexpression of a glycosyltransferase, which may lead to Golgi stress.

Related Articles

Journal Cover

July 2011
Volume 39 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kawamoto S, Moriwaki K, Nakagawa T, Terao M, Shinzaki S, Yamane-Ohnuki N, Satoh M, Mehta AS, Block TM, Miyoshi E, Miyoshi E, et al: Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner. Int J Oncol 39: 203-208, 2011
APA
Kawamoto, S., Moriwaki, K., Nakagawa, T., Terao, M., Shinzaki, S., Yamane-Ohnuki, N. ... Miyoshi, E. (2011). Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner. International Journal of Oncology, 39, 203-208. https://doi.org/10.3892/ijo.2011.1005
MLA
Kawamoto, S., Moriwaki, K., Nakagawa, T., Terao, M., Shinzaki, S., Yamane-Ohnuki, N., Satoh, M., Mehta, A. S., Block, T. M., Miyoshi, E."Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner". International Journal of Oncology 39.1 (2011): 203-208.
Chicago
Kawamoto, S., Moriwaki, K., Nakagawa, T., Terao, M., Shinzaki, S., Yamane-Ohnuki, N., Satoh, M., Mehta, A. S., Block, T. M., Miyoshi, E."Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner". International Journal of Oncology 39, no. 1 (2011): 203-208. https://doi.org/10.3892/ijo.2011.1005