INTERLEUKIN-1 RECEPTOR ANTAGONIST INHIBITS GROWTH MODULATION OF HUMAN TUMOR-CELL LINES BY INTERLEUKIN-1 IN-VITRO

  • Authors:
    • E OELMANN
    • MS TOPP
    • B REUFI
    • C PAPADIMITRIOU
    • M KOENIGSMANN
    • D OBERBERG
    • E THIEL
    • WE BERDEL
  • View Affiliations

  • Published online on: March 1, 1994     https://doi.org/10.3892/ijo.4.3.555
  • Pages: 555-558
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Abstract

In this study we report that recombinant human (rh) interleukin-1alpha (IL-1alpha) has direct and dose-dependent growth-modulating effects on human tumor cell lines in vitro as measured by a human tumor cloning assay (HTCA). Colony formation of the melanoma cell line A 375 was inhibited by rhIL-1alpha, whereas colony formation of the glioblastoma cell line HTB 14 was enhanced by this cytokine. Both growth-modulating effects were dose-dependent, however with some saturation. Subsequently, we have tested the activity of recombinant human IL-1 receptor antagonist (rhIL-1ra) on the tumor growth modulation by rhIL-1alpha. Tumor cells were incubated with increasing concentrations of rhIL-1ra and then added to the cultures containing rHIL-1alpha. Concentrations of rhIL-1ra were chosen to achieve a range between 0.01 and 100 ng/ml which includes a 1000-fold molar excess over IL-1alpha. The receptor antagonist was able to block both the inhibition and the stimulation of clonal growth of the respective tumor cell line by rhIL-1alpha. Furthermore, there was a direct dose dependent relationship revealing higher IL-1 antagonism of rhIL-1ra at higher concentrations with maximum efficacy at 1000-molar excess concentrations over IL-1alpha. In addition, rhIL-1ra alone did not reveal major modulation of the growth of A 375, but significantly decreased colony formation of HTB 14. We conclude that rhIL-1ra can counteract modulation of clonal growth of human tumor cells by IL-1alpha in vitro. Since our report provides first evidence that the stimulation of clonal tumor cell growth by IL-1alpha can be blocked by rhIL-1ra, this member of the IL-1 cytokine network should be further studied as a possible candidate for experimental cancer treatment.

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March 1994
Volume 4 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
OELMANN E, TOPP M, REUFI B, PAPADIMITRIOU C, KOENIGSMANN M, OBERBERG D, THIEL E and BERDEL W: INTERLEUKIN-1 RECEPTOR ANTAGONIST INHIBITS GROWTH MODULATION OF HUMAN TUMOR-CELL LINES BY INTERLEUKIN-1 IN-VITRO. Int J Oncol 4: 555-558, 1994
APA
OELMANN, E., TOPP, M., REUFI, B., PAPADIMITRIOU, C., KOENIGSMANN, M., OBERBERG, D. ... BERDEL, W. (1994). INTERLEUKIN-1 RECEPTOR ANTAGONIST INHIBITS GROWTH MODULATION OF HUMAN TUMOR-CELL LINES BY INTERLEUKIN-1 IN-VITRO. International Journal of Oncology, 4, 555-558. https://doi.org/10.3892/ijo.4.3.555
MLA
OELMANN, E., TOPP, M., REUFI, B., PAPADIMITRIOU, C., KOENIGSMANN, M., OBERBERG, D., THIEL, E., BERDEL, W."INTERLEUKIN-1 RECEPTOR ANTAGONIST INHIBITS GROWTH MODULATION OF HUMAN TUMOR-CELL LINES BY INTERLEUKIN-1 IN-VITRO". International Journal of Oncology 4.3 (1994): 555-558.
Chicago
OELMANN, E., TOPP, M., REUFI, B., PAPADIMITRIOU, C., KOENIGSMANN, M., OBERBERG, D., THIEL, E., BERDEL, W."INTERLEUKIN-1 RECEPTOR ANTAGONIST INHIBITS GROWTH MODULATION OF HUMAN TUMOR-CELL LINES BY INTERLEUKIN-1 IN-VITRO". International Journal of Oncology 4, no. 3 (1994): 555-558. https://doi.org/10.3892/ijo.4.3.555