CHARACTERIZATION OF ADULT HUMAN PROSTATIC EPITHELIAL-CELLS IMMORTALIZED BY POLYBRENE-INDUCED DNA TRANSFECTION WITH A PLASMID CONTAINING AN ORIGIN-DEFECTIVE SV40-GENOME

  • Authors:
    • MS LEE
    • E GARKOVENKO
    • JS YUN
    • PC WEIJERMAN
    • DM PEEHL
    • LS CHEN
    • JS RHIM
  • View Affiliations

  • Published online on: April 1, 1994     https://doi.org/10.3892/ijo.4.4.821
  • Pages: 821-830
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Abstract

Normal adult human prostatic epithelial cells were infected with an adenovirus 12-SV40 virus or transfected by polybrene-induced gene transfer with a plasmid (pRSV-T) containing the SV40 early region genes or with a plasmid (pRNS-1) containing an origin-defective SV40 genome and a plasmid carrying the neomycin resistance gene. Colonies of morphologically altered cells were isolated, cultured in a serum-free medium and characterized. These cells had extended lifespan in culture compared to normal adult human prostatic epithelial cells. Both Ad12-SV40-infected and pRSV-T-transfected cultures eventually underwent senescence. pRNS-1-transfected cells (pRNS-1-1), however, have now been grown for more than 50 passages. These cells contain the SV40 genome, express SV40 T-antigen, and are not tumorigenic in nude mice. They express cytokeratins 5 and 8, like the parent cells, and are pseudodiploid. Analysis of growth regulatory processes revealed that the growth of pRNS-1-1 cells was stimulated similarly to that of normal prostatic epithelial cells by epidermal growth factor, insulin-like growth factor, and pituitary extract. The response of pRNS-1 cells to a growth-inhibitory factor, retinoic acid, was also similar to that of normal cells. However, pRNS-1-1 cells were less responsive than normal cells to growth inhibition by transforming growth factor-beta, and had lost altogether the ability of normal cells to be inhibited by tumor necrosis factor-alpha and 1,25 (OH)2 vitamin D3. Therefore transformation appeared to alter growth-inhibitory but not growth - stimulatory mechanisms. These cells should be useful in elucidating the multistep mechanism of carcinogenesis of the prostate.

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April 1994
Volume 4 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
LEE M, GARKOVENKO E, YUN J, WEIJERMAN P, PEEHL D, CHEN L and RHIM J: CHARACTERIZATION OF ADULT HUMAN PROSTATIC EPITHELIAL-CELLS IMMORTALIZED BY POLYBRENE-INDUCED DNA TRANSFECTION WITH A PLASMID CONTAINING AN ORIGIN-DEFECTIVE SV40-GENOME. Int J Oncol 4: 821-830, 1994
APA
LEE, M., GARKOVENKO, E., YUN, J., WEIJERMAN, P., PEEHL, D., CHEN, L., & RHIM, J. (1994). CHARACTERIZATION OF ADULT HUMAN PROSTATIC EPITHELIAL-CELLS IMMORTALIZED BY POLYBRENE-INDUCED DNA TRANSFECTION WITH A PLASMID CONTAINING AN ORIGIN-DEFECTIVE SV40-GENOME. International Journal of Oncology, 4, 821-830. https://doi.org/10.3892/ijo.4.4.821
MLA
LEE, M., GARKOVENKO, E., YUN, J., WEIJERMAN, P., PEEHL, D., CHEN, L., RHIM, J."CHARACTERIZATION OF ADULT HUMAN PROSTATIC EPITHELIAL-CELLS IMMORTALIZED BY POLYBRENE-INDUCED DNA TRANSFECTION WITH A PLASMID CONTAINING AN ORIGIN-DEFECTIVE SV40-GENOME". International Journal of Oncology 4.4 (1994): 821-830.
Chicago
LEE, M., GARKOVENKO, E., YUN, J., WEIJERMAN, P., PEEHL, D., CHEN, L., RHIM, J."CHARACTERIZATION OF ADULT HUMAN PROSTATIC EPITHELIAL-CELLS IMMORTALIZED BY POLYBRENE-INDUCED DNA TRANSFECTION WITH A PLASMID CONTAINING AN ORIGIN-DEFECTIVE SV40-GENOME". International Journal of Oncology 4, no. 4 (1994): 821-830. https://doi.org/10.3892/ijo.4.4.821