EFFECT OF 12-DEOXYPHORBOL 13-PHENYLACETATE ON DAUNORUBICIN RESISTANCE AND CALCIUM-INDEPENDENT PROTEIN-KINASE-C ISOZYMES IN DRUG-SENSITIVE MURINE LEUKEMIA P388 CELLS

  • Authors:
    • S GOLLAPUDI
    • H SINGH
    • S GUPTA
  • View Affiliations

  • Published online on: April 1, 1994     https://doi.org/10.3892/ijo.4.4.849
  • Pages: 849-851
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Because of a suggested role of protein kinase C (PKC) in multidrug resistance (MDR) and recent. demonstration of molecular and biochemical heterogeneity of PKC, we examined the effect of 12-deoxyphorbol 13-phenylacetate (dPP) on intracellular accumulation and drug sensitivity of daunorubicin (DNR) in drug sensitive P388 murine leukemia cell line. In addition, effect of dPP on Ca++-independent PKC (delta, epsilon and zeta) isozymes was examined with Western blot, using laser densitometry. dPP induced significant resistance to DNR in P388 cells, however had no significant effect on DNR accumulation. In addition, dPP increased the levels of plasma membrane associated PKCdelta and epsilon isozymes in P388 cells and but had no effect on plasma membrane-associated PKCzeta isozyme. This study demonstrates a role of Ca++-independent PKCdelta and epsilon isozymes in drug resistance.

Related Articles

Journal Cover

April 1994
Volume 4 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
GOLLAPUDI S, SINGH H and GUPTA S: EFFECT OF 12-DEOXYPHORBOL 13-PHENYLACETATE ON DAUNORUBICIN RESISTANCE AND CALCIUM-INDEPENDENT PROTEIN-KINASE-C ISOZYMES IN DRUG-SENSITIVE MURINE LEUKEMIA P388 CELLS. Int J Oncol 4: 849-851, 1994
APA
GOLLAPUDI, S., SINGH, H., & GUPTA, S. (1994). EFFECT OF 12-DEOXYPHORBOL 13-PHENYLACETATE ON DAUNORUBICIN RESISTANCE AND CALCIUM-INDEPENDENT PROTEIN-KINASE-C ISOZYMES IN DRUG-SENSITIVE MURINE LEUKEMIA P388 CELLS. International Journal of Oncology, 4, 849-851. https://doi.org/10.3892/ijo.4.4.849
MLA
GOLLAPUDI, S., SINGH, H., GUPTA, S."EFFECT OF 12-DEOXYPHORBOL 13-PHENYLACETATE ON DAUNORUBICIN RESISTANCE AND CALCIUM-INDEPENDENT PROTEIN-KINASE-C ISOZYMES IN DRUG-SENSITIVE MURINE LEUKEMIA P388 CELLS". International Journal of Oncology 4.4 (1994): 849-851.
Chicago
GOLLAPUDI, S., SINGH, H., GUPTA, S."EFFECT OF 12-DEOXYPHORBOL 13-PHENYLACETATE ON DAUNORUBICIN RESISTANCE AND CALCIUM-INDEPENDENT PROTEIN-KINASE-C ISOZYMES IN DRUG-SENSITIVE MURINE LEUKEMIA P388 CELLS". International Journal of Oncology 4, no. 4 (1994): 849-851. https://doi.org/10.3892/ijo.4.4.849