INTERPHASE CYTOGENETIC ANALYSIS OF CULTURED PLEOMORPHIC ADENOMAS WITH A NORMAL KARYOTYPE

  • Authors:
    • P SAHLIN
    • J MARK
    • G STENMAN
  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1225
  • Pages: 1225-1228
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Abstract

A subgroup of pleomorphic adenomas of the salivary glands is characterized by apparently normal stemline karyotypes. In this investigation we have used FISH to study whether clonal translocations involving chromosomes 8 and 12 might have escaped detection with conventional cytogenetic analysis. Whole chromosome painting probes for chromosomes 8 and 12 were hybridized to interphase nuclei from 22 cytogenetically normal pleomorphic adenomas. In 20 of the 22 cases the majority of the nuclei (75-93%) showed two hybridizing chromosome 8 and chromosome 12 domains of equal size, indicating the presence of two intact copies of each of these chromosomes. Evidence of clonal translocations was only detected in two tumors. These findings show that in the vast majority of adenomas with a normal karyotype we can exclude the possibility of an abnormal non-dividing or very slow growing, and thus undetected, tumor cell population in vitro. Our data provides further evidence to support the existence of a subgroup of pleomorphic adenomas with a normal karyotype.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
SAHLIN P, MARK J and STENMAN G: INTERPHASE CYTOGENETIC ANALYSIS OF CULTURED PLEOMORPHIC ADENOMAS WITH A NORMAL KARYOTYPE. Int J Oncol 4: 1225-1228, 1994
APA
SAHLIN, P., MARK, J., & STENMAN, G. (1994). INTERPHASE CYTOGENETIC ANALYSIS OF CULTURED PLEOMORPHIC ADENOMAS WITH A NORMAL KARYOTYPE. International Journal of Oncology, 4, 1225-1228. https://doi.org/10.3892/ijo.4.6.1225
MLA
SAHLIN, P., MARK, J., STENMAN, G."INTERPHASE CYTOGENETIC ANALYSIS OF CULTURED PLEOMORPHIC ADENOMAS WITH A NORMAL KARYOTYPE". International Journal of Oncology 4.6 (1994): 1225-1228.
Chicago
SAHLIN, P., MARK, J., STENMAN, G."INTERPHASE CYTOGENETIC ANALYSIS OF CULTURED PLEOMORPHIC ADENOMAS WITH A NORMAL KARYOTYPE". International Journal of Oncology 4, no. 6 (1994): 1225-1228. https://doi.org/10.3892/ijo.4.6.1225