A NOVEL V-ERB-B-TRANSFORMED CELL-LINE CO-EXPRESSING LYMPHOID AND MYELOID SURFACE-MARKERS

  • Authors:
    • M MILLER
    • G SYMONDS
  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1277
  • Pages: 1277-1281
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Abstract

We have generated a novel hematopoietic cell line which co-expresses lymphoid and myeloid cell surface markers. This cell line, termed E6-2TH, was derived from thymus tissue of a mouse which developed leukemia following lethal irradiation and reconstitution with retrovirally infected bone marrow progenitor cells. Infection was effected with a recombinant murine retrovirus containing v-erb-B. The cells which grew out from the thymus tissue were initially dependent on WEHI-3B conditioned medium as a source of exogenous growth factor, but progressed in culture to become growth factor independent. Flow cytometric analysis using lineage-specific surface markers demonstrated that the initial growth factor dependent cells co-expressed myeloid and B lymphoid antigens, while the derived growth factor independent cells expressed only antigens specific to the B lymphoid lineage. This result indicates a mixed lymphoid/myeloid phenotype in this cell line and subsequent lineage specialisation during in vitro tumor progression.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
MILLER M and MILLER M: A NOVEL V-ERB-B-TRANSFORMED CELL-LINE CO-EXPRESSING LYMPHOID AND MYELOID SURFACE-MARKERS. Int J Oncol 4: 1277-1281, 1994
APA
MILLER, M., & MILLER, M. (1994). A NOVEL V-ERB-B-TRANSFORMED CELL-LINE CO-EXPRESSING LYMPHOID AND MYELOID SURFACE-MARKERS. International Journal of Oncology, 4, 1277-1281. https://doi.org/10.3892/ijo.4.6.1277
MLA
MILLER, M., SYMONDS, G."A NOVEL V-ERB-B-TRANSFORMED CELL-LINE CO-EXPRESSING LYMPHOID AND MYELOID SURFACE-MARKERS". International Journal of Oncology 4.6 (1994): 1277-1281.
Chicago
MILLER, M., SYMONDS, G."A NOVEL V-ERB-B-TRANSFORMED CELL-LINE CO-EXPRESSING LYMPHOID AND MYELOID SURFACE-MARKERS". International Journal of Oncology 4, no. 6 (1994): 1277-1281. https://doi.org/10.3892/ijo.4.6.1277