MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma

  • Authors:
    • Yuan Tian
    • Yang Nan
    • Lei Han
    • Anling Zhang
    • Guangxiu Wang
    • Zhifan Jia
    • Jianwei Hao
    • Peiyu Pu
    • Yue Zhong
    • Chunsheng Kang
  • View Affiliations

  • Published online on: December 15, 2011     https://doi.org/10.3892/ijo.2011.1306
  • Pages: 1105-1112
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Abstract

The microRNA miR-451 is downregulated in gliomas, this has been suggested by several different research groups and is consistent with our data. Our previous study also confirmed that miR-451 has a repressive role in glioma by inhibiting cell growth, proliferation and by inducing cell apoptosis. In the present study, we identified a target gene of miR-451 in human glioma and investigated the mechanism for the glioma suppressive effect of miR-451 functions. Expression of miR-451 in gliomas was identified by quantitative real-time PCR and fluorescence in situ hybridization. Human glioma cell lines (U251, U87, LN229 and A172) were transfected with miR-451 mimics to restore miR-451 expression. The tumor suppressive effects of miR-451 were further verified by subcutaneous assays in nude mice, in addition to our previous in vitro data. A candidate target gene was tested by Western blotting and luciferase reporter assays. Some PI3K/AKT pathway factors were tested by Western blotting. We found that miR-451 expression was downregulated in glioma samples and was inversely correlated with WHO grades of gliomas. In vivo assays confirmed that miR-451 had tumor suppressive traits. CAB39-3'UTR lucife-rase reporter assay confirmed CAB39 as a direct target gene of miR-451. Significant alterations in the expression of PI3K/AKT pathway factors were observed by Western blot assays. We conclude that miR-451 represses glioma in vitro and in vivo, likely through targeting CAB39 directly and inhibiting the PI3K/AKT pathway indirectly.
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April 2012
Volume 40 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tian Y, Nan Y, Han L, Zhang A, Wang G, Jia Z, Hao J, Pu P, Zhong Y, Kang C, Kang C, et al: MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma. Int J Oncol 40: 1105-1112, 2012
APA
Tian, Y., Nan, Y., Han, L., Zhang, A., Wang, G., Jia, Z. ... Kang, C. (2012). MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma. International Journal of Oncology, 40, 1105-1112. https://doi.org/10.3892/ijo.2011.1306
MLA
Tian, Y., Nan, Y., Han, L., Zhang, A., Wang, G., Jia, Z., Hao, J., Pu, P., Zhong, Y., Kang, C."MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma". International Journal of Oncology 40.4 (2012): 1105-1112.
Chicago
Tian, Y., Nan, Y., Han, L., Zhang, A., Wang, G., Jia, Z., Hao, J., Pu, P., Zhong, Y., Kang, C."MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma". International Journal of Oncology 40, no. 4 (2012): 1105-1112. https://doi.org/10.3892/ijo.2011.1306