Pinus densiflora leaf essential oil induces apoptosis via ROS generation and activation of caspases in YD-8 human oral cancer cells

  • Authors:
    • Jeong-Rang Jo
    • Ju Sung Park
    • Yu-Kyοung Park
    • Young Zoo Chae
    • Gyu-Hee Lee
    • Gy-Young Park
    • Byeong-Churl Jang
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  • Published online on: November 11, 2011     https://doi.org/10.3892/ijo.2011.1263
  • Pages: 1238-1245
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Abstract

The leaf of Pinus (P.) densiflora, a pine tree widely distributed in Asian countries, has been used as a traditional medicine. In the present study, we investigated the anticancer activity of essential oil, extracted by steam distillation, from the leaf of P. densiflora in YD-8 human oral squamous cell carcinoma (OSCC) cells. Treatment of YD-8 cells with P. densiflora leaf essential oil (PLEO) at 60 µg/ml for 8 h strongly inhibited proliferation and survival and induced apoptosis. Notably, treatment with PLEO led to generation of ROS, activation of caspase-9, PARP cleavage, down-regulation of Bcl-2, and phosphorylation of ERK-1/2 and JNK-1/2 in YD-8 cells. Treatment with PLEO, however, did not affect the expression of Bax, XIAP and GRP78. Importantly, pharmaco­logical inhibition studies demonstrated that treatment with vitamin E (an anti-oxidant) or z-VAD-fmk (a pan-caspase inhibitor), but not with PD98059 (an ERK-1/2 inhibitor) or SP600125 (a JNK-1/2 inhibitor), strongly suppressed PLEO-induced apoptosis in YD-8 cells and reduction of their survival. Vitamin E treatment further blocked activation of caspase-9 and Bcl-2 down-regulation induced by PLEO. Thus, these results demonstrate firstly that PLEO has anti-proliferative, anti-survival and pro-apoptotic effects on YD-8 cells and the effects are largely due to the ROS-dependent activation of caspases.
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April 2012
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Spandidos Publications style
Jo J, Park JS, Park Y, Chae YZ, Lee G, Park G and Jang B: Pinus densiflora leaf essential oil induces apoptosis via ROS generation and activation of caspases in YD-8 human oral cancer cells. Int J Oncol 40: 1238-1245, 2012
APA
Jo, J., Park, J.S., Park, Y., Chae, Y.Z., Lee, G., Park, G., & Jang, B. (2012). Pinus densiflora leaf essential oil induces apoptosis via ROS generation and activation of caspases in YD-8 human oral cancer cells. International Journal of Oncology, 40, 1238-1245. https://doi.org/10.3892/ijo.2011.1263
MLA
Jo, J., Park, J. S., Park, Y., Chae, Y. Z., Lee, G., Park, G., Jang, B."Pinus densiflora leaf essential oil induces apoptosis via ROS generation and activation of caspases in YD-8 human oral cancer cells". International Journal of Oncology 40.4 (2012): 1238-1245.
Chicago
Jo, J., Park, J. S., Park, Y., Chae, Y. Z., Lee, G., Park, G., Jang, B."Pinus densiflora leaf essential oil induces apoptosis via ROS generation and activation of caspases in YD-8 human oral cancer cells". International Journal of Oncology 40, no. 4 (2012): 1238-1245. https://doi.org/10.3892/ijo.2011.1263