Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance

  • Authors:
    • Yojiro Hashiguchi
    • Naohiro Nishida
    • Koshi Mimori
    • Tomoya Sudo
    • Fumiaki Tanaka
    • Kohei Shibata
    • Hideshi Ishii
    • Hidetaka Mochizuki
    • Kazuo Hase
    • Yuichiro Doki
    • Masaki Mori
  • View Affiliations

  • Published online on: February 9, 2012     https://doi.org/10.3892/ijo.2012.1363
  • Pages: 1477-1482
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Abstract

Recent reports have demonstrated that another strand of mature microRNA (miRNA), called microRNA* or 3p (5p) strand, which is generated from the same precursor miRNA (Pre-miR), has a crucial role in cellular function. We previously reported the tumor suppressive effect of miR-125a-5p in gastric cancer. The current study was designed to examine the function and clinical significance of miR-125a-3p, a partner strand of miR-125a-5p, in human gastric cancer. Quantitative RT-PCR was used to evaluate miR-125a-3p expression in 70 gastric cancer cases to determine the clinicopathologic significance of miR-125a-3p expression. In addition, the effect of miR-125a-3p on the proliferation of gastric cancer cells was investigated. Low expression levels of miR-125a-3p were associated with indicators of enhanced malignant potential such as tumor size (p=0.0002), tumor invasion (p=0.0149), lymph node metastasis (p=0.018), liver metastasis (p=0.016), peritoneal dissemination (p=0.03), advanced clinical stage (p=0.0037) and poor prognosis (p=0.0083). Multivariate analysis indicated that low miR-125a-3p expression was an independent prognostic factor for survival, while in vitro assays demonstrated that miR‑125a-3p suppressed the proliferation of gastric cancer cells. MiR-125a-3p is a potent prognostic marker in gastric cancer. The clinical significance and tumor suppressive effect of miR‑125a-3p, as well as previously reported miR-125a-5p, suggest that the functional role of another strand of the mature form miRNA cannot be ignored, at least in miR-125a biogenesis.

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May 2012
Volume 40 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hashiguchi Y, Nishida N, Mimori K, Sudo T, Tanaka F, Shibata K, Ishii H, Mochizuki H, Hase K, Doki Y, Doki Y, et al: Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance. Int J Oncol 40: 1477-1482, 2012
APA
Hashiguchi, Y., Nishida, N., Mimori, K., Sudo, T., Tanaka, F., Shibata, K. ... Mori, M. (2012). Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance. International Journal of Oncology, 40, 1477-1482. https://doi.org/10.3892/ijo.2012.1363
MLA
Hashiguchi, Y., Nishida, N., Mimori, K., Sudo, T., Tanaka, F., Shibata, K., Ishii, H., Mochizuki, H., Hase, K., Doki, Y., Mori, M."Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance". International Journal of Oncology 40.5 (2012): 1477-1482.
Chicago
Hashiguchi, Y., Nishida, N., Mimori, K., Sudo, T., Tanaka, F., Shibata, K., Ishii, H., Mochizuki, H., Hase, K., Doki, Y., Mori, M."Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance". International Journal of Oncology 40, no. 5 (2012): 1477-1482. https://doi.org/10.3892/ijo.2012.1363