Expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and TIMP3 by hepatocellular carcinoma cell lines

  • Authors:
    • Sharon L. Turner
    • David Mangnall
    • Nigel C. Bird
    • Rowena A.D. Bunning
    • Maria E. Blair-Zajdel
  • View Affiliations

  • Published online on: June 20, 2012     https://doi.org/10.3892/ijo.2012.1525
  • Pages: 1043-1049
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Abstract

Little is known about the expression or role of ADAMTS-1, -4 and -5 and their endogenous inhibitor TIMP3 in the liver in physiological and pathological conditions. Their expression was, therefore, investigated in the hepatocellular carcinoma cell lines HepG2 and HuH-7 using qRT-PCR and western blotting, and their cellular localisation by immunocytochemistry. Cytokine treatments were used to assess mRNA and protein modulation. ADAMTS-1, -4, -5 and TIMP3 mRNA and protein were detected in both HepG2 and HuH-7 cells. IL-1β and IL-6 treatments significantly modulated ADAMTS-1 mRNA expression and IL-1β treatment ADAMTS-4 mRNA expression in HepG2 cells. Modulations of mRNA by ≥5-fold did not translate to increased protein expression. This study showed that ADAMTS-1, -4, -5 and TIMP3 were expressed at differential levels in hepatocellular carcinoma cell lines. The pro-inflammatory cytokines IL-1β, TNF-α or IL-6 induced changes in mRNA expression, although these did not translate to the protein level.
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September 2012
Volume 41 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Turner SL, Mangnall D, Bird NC, Bunning RA and Blair-Zajdel ME: Expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and TIMP3 by hepatocellular carcinoma cell lines. Int J Oncol 41: 1043-1049, 2012
APA
Turner, S.L., Mangnall, D., Bird, N.C., Bunning, R.A., & Blair-Zajdel, M.E. (2012). Expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and TIMP3 by hepatocellular carcinoma cell lines. International Journal of Oncology, 41, 1043-1049. https://doi.org/10.3892/ijo.2012.1525
MLA
Turner, S. L., Mangnall, D., Bird, N. C., Bunning, R. A., Blair-Zajdel, M. E."Expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and TIMP3 by hepatocellular carcinoma cell lines". International Journal of Oncology 41.3 (2012): 1043-1049.
Chicago
Turner, S. L., Mangnall, D., Bird, N. C., Bunning, R. A., Blair-Zajdel, M. E."Expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and TIMP3 by hepatocellular carcinoma cell lines". International Journal of Oncology 41, no. 3 (2012): 1043-1049. https://doi.org/10.3892/ijo.2012.1525