Parthenolide suppresses tumor growth in a xenograft model of colorectal cancer cells by inducing mitochondrial dysfunction and apoptosis

  • Authors:
    • Se-Lim Kim
    • Kieu The Thu Trang
    • Seong Hun Kim
    • In Hee Kim
    • Seung Ok Lee
    • Soo Teik Lee
    • Dae Ghon Kim
    • Sang-Wook Kim
  • View Affiliations

  • Published online on: August 9, 2012     https://doi.org/10.3892/ijo.2012.1587
  • Pages: 1547-1553
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Abstract

Parthenolide (PT), a principal active component in medicinal plants, has been used conventionally to treat migraine and inflammation. This component has recently been reported to induce apoptosis in cancer cells, through mitochondrial dysfunction. In the present study, we investigated PT-mediated cell death signaling pathway by focusing on the involvement of Bcl-2 family members in human colorectal cancer cells. We also investigated the inhibitory effect of PT on tumor growth in xenografts. Using the human colorectal cancer cell lines HT-29, SW620 and LS174T, we demonstrated that treatment of these cancer cells with PT induces apoptosis using MTT, Annexin V assay and Hoechst 33258 staining. Apoptosis through the mitochondrial pathway was confirmed by detecting regulation of Bcl-2 family members, cytochrome c release and caspase activation. Moreover, intraperitoneal injection of PT showed significant inhibition of tumor growth, angiogenesis in the xenograft model. These results demonstrate that PT exhibits anti-cancer activity in human colorectal cancer in vitro and in vivo. These findings may also provide a novel approach for the treatment of colorectal cancer.
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October 2012
Volume 41 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim S, Trang KT, Kim SH, Kim IH, Lee SO, Lee ST, Kim DG and Kim S: Parthenolide suppresses tumor growth in a xenograft model of colorectal cancer cells by inducing mitochondrial dysfunction and apoptosis. Int J Oncol 41: 1547-1553, 2012
APA
Kim, S., Trang, K.T., Kim, S.H., Kim, I.H., Lee, S.O., Lee, S.T. ... Kim, S. (2012). Parthenolide suppresses tumor growth in a xenograft model of colorectal cancer cells by inducing mitochondrial dysfunction and apoptosis. International Journal of Oncology, 41, 1547-1553. https://doi.org/10.3892/ijo.2012.1587
MLA
Kim, S., Trang, K. T., Kim, S. H., Kim, I. H., Lee, S. O., Lee, S. T., Kim, D. G., Kim, S."Parthenolide suppresses tumor growth in a xenograft model of colorectal cancer cells by inducing mitochondrial dysfunction and apoptosis". International Journal of Oncology 41.4 (2012): 1547-1553.
Chicago
Kim, S., Trang, K. T., Kim, S. H., Kim, I. H., Lee, S. O., Lee, S. T., Kim, D. G., Kim, S."Parthenolide suppresses tumor growth in a xenograft model of colorectal cancer cells by inducing mitochondrial dysfunction and apoptosis". International Journal of Oncology 41, no. 4 (2012): 1547-1553. https://doi.org/10.3892/ijo.2012.1587