Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA

  • Authors:
    • Akiko Kawai
    • Toshiyuki Kobayashi
    • Okio Hino
  • View Affiliations

  • Published online on: March 21, 2013     https://doi.org/10.3892/ijo.2013.1862
  • Pages: 1597-1604
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Abstract

Birt-Hogg-Dubé syndrome (BHDS) is an autosomal dominantly inherited disease characterized by spontaneous pneumothorax, hair folliculomas and renal tumors. The responsible gene, BHD, is a tumor suppressor and encodes folliculin. Folliculin is an evolutionarily conserved protein (~67 kDa) with no apparent functional motif and its role has not yet been fully elucidated. In this study, we found that knockdown of BHD increased the levels of cyclin D1 in HeLa cells. A reporter assay with the cyclin D1 gene (CCND1) promoter region indicated that this increase was not caused by activation of transcription through known cis-acting elements. We examined the possibility of post-transcriptional mechanism using reporter constructs containing fragments of the cyclin D1 3' untranslated region (3'UTR). Transfection of control cells with a construct carrying a medial 1.3 kb 3'UTR fragment resulted in a significant reduction in luciferase activity. This effect was largely prevented by knockdown of BHD. Our results suggest that the post-transcriptional regulation of the CCND1 expression by BHD may be associated with microRNA(s) or RNA binding protein(s) that bind to the 3'UTR.
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May 2013
Volume 42 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kawai A, Kobayashi T and Hino O: Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA. Int J Oncol 42: 1597-1604, 2013
APA
Kawai, A., Kobayashi, T., & Hino, O. (2013). Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA. International Journal of Oncology, 42, 1597-1604. https://doi.org/10.3892/ijo.2013.1862
MLA
Kawai, A., Kobayashi, T., Hino, O."Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA". International Journal of Oncology 42.5 (2013): 1597-1604.
Chicago
Kawai, A., Kobayashi, T., Hino, O."Folliculin regulates cyclin D1 expression through cis-acting elements in the 3' untranslated region of cyclin D1 mRNA". International Journal of Oncology 42, no. 5 (2013): 1597-1604. https://doi.org/10.3892/ijo.2013.1862