The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins

  • Authors:
    • Jing Nan Lu
    • Won Sup Lee
    • Min Jeong Kim
    • Jeong Won Yun
    • Ji Hyun Jung
    • Sang Mi Yi
    • Jae-Hoon Jeong
    • Hye Jung Kim
    • Yung Hyun Choi
    • Gon‑Sup Kim
    • Chung Ho Ryu
    • Sung Chul Shin
  • View Affiliations

  • Published online on: February 27, 2014     https://doi.org/10.3892/ijo.2014.2315
  • Pages: 1756-1766
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Abstract

Evidence suggests that anthocyanins inhibit EGFR and Akt activity. However, it is still unknown whether the inhibitory effect of anthocyanins on Akt is associated with the anti-EGFR effect. The effect of anthocyanins on epithelial-mesenchymal transition (EMT) has not been extensively studied. Therefore, we investigated the effects of anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) on EGF-induced EMT and the underlying molecular mechanisms. AIMs suppressed the invasion of A549 cells in a dose-dependent manner. AIMs inhibited the phosphorylation of Akt and EGFR, but the inhibitory effect on Akt was not derived from EGFR. EGF re-induced Akt phosphorylation at Thr308 in the AIM-treated cells, but not Akt phosphorylation at Ser473. AIMs also inhibited EMT of cancer cells. AIMs inhibited glycogen synthase kinase-3β phosphorylation and β-catenin expression that are invovled in EMT. We confirmed these findings with transforming growth factor (TGF)-β. In conclusion, these data suggest that the inhibitory effect of AIMs on Akt activity is independent of EGFR, and that AIMs suppressed invasion and migration at least in part by suppressing EMT by inhibiting Akt activity as well as EGFR. This study provides evidence that AIMs may have anticancer effects on human cancer cells.
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May-2014
Volume 44 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lu JN, Lee WS, Kim MJ, Yun JW, Jung JH, Yi SM, Jeong J, Kim HJ, Choi YH, Kim GS, Kim GS, et al: The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins. Int J Oncol 44: 1756-1766, 2014
APA
Lu, J.N., Lee, W.S., Kim, M.J., Yun, J.W., Jung, J.H., Yi, S.M. ... Shin, S.C. (2014). The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins. International Journal of Oncology, 44, 1756-1766. https://doi.org/10.3892/ijo.2014.2315
MLA
Lu, J. N., Lee, W. S., Kim, M. J., Yun, J. W., Jung, J. H., Yi, S. M., Jeong, J., Kim, H. J., Choi, Y. H., Kim, G., Ryu, C. H., Shin, S. C."The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins". International Journal of Oncology 44.5 (2014): 1756-1766.
Chicago
Lu, J. N., Lee, W. S., Kim, M. J., Yun, J. W., Jung, J. H., Yi, S. M., Jeong, J., Kim, H. J., Choi, Y. H., Kim, G., Ryu, C. H., Shin, S. C."The inhibitory effect of anthocyanins on Akt on invasion and epithelial-mesenchymal transition is not associated with the anti-EGFR effect of the anthocyanins". International Journal of Oncology 44, no. 5 (2014): 1756-1766. https://doi.org/10.3892/ijo.2014.2315