Potential crosstalk of Ca2+-ROS-dependent mechanism involved in apoptosis of Kasumi-1 cells mediated by heme oxygenase-1 small interfering RNA

  • Authors:
    • Sixi Wei
    • Yating Wang
    • Qixiang Chai
    • Qin Fang
    • Yaming Zhang
    • Jishi Wang
  • View Affiliations

  • Published online on: September 17, 2014     https://doi.org/10.3892/ijo.2014.2661
  • Pages: 2373-2384
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Abstract

Acute myeloid leukemia (AML) requires new therapies on the molecular level. Downregulation of heme oxygenase-1 (HO-1) by gene silencing improves the sensitivity of tumor cells to chemotherapy drugs and promotes apoptosis. For the first time, we verified that endoplasmic reticulum and mitochondrial apoptotic pathways were activated by small interfering RNA that targeted-silenced the expression of HO-1 in AML-M2 Kasumi-1 cells. Ca2+ was prone to accumulation and reactive oxygen species were easily generated, while mitochondrial transmembrane potential was reduced. Thus, cytochrome c was released from mitochondria to the cytoplasm and caspases were activated for the following cascade to facilitate apoptosis.

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December-2014
Volume 45 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wei S, Wang Y, Chai Q, Fang Q, Zhang Y and Wang J: Potential crosstalk of Ca2+-ROS-dependent mechanism involved in apoptosis of Kasumi-1 cells mediated by heme oxygenase-1 small interfering RNA. Int J Oncol 45: 2373-2384, 2014
APA
Wei, S., Wang, Y., Chai, Q., Fang, Q., Zhang, Y., & Wang, J. (2014). Potential crosstalk of Ca2+-ROS-dependent mechanism involved in apoptosis of Kasumi-1 cells mediated by heme oxygenase-1 small interfering RNA. International Journal of Oncology, 45, 2373-2384. https://doi.org/10.3892/ijo.2014.2661
MLA
Wei, S., Wang, Y., Chai, Q., Fang, Q., Zhang, Y., Wang, J."Potential crosstalk of Ca2+-ROS-dependent mechanism involved in apoptosis of Kasumi-1 cells mediated by heme oxygenase-1 small interfering RNA". International Journal of Oncology 45.6 (2014): 2373-2384.
Chicago
Wei, S., Wang, Y., Chai, Q., Fang, Q., Zhang, Y., Wang, J."Potential crosstalk of Ca2+-ROS-dependent mechanism involved in apoptosis of Kasumi-1 cells mediated by heme oxygenase-1 small interfering RNA". International Journal of Oncology 45, no. 6 (2014): 2373-2384. https://doi.org/10.3892/ijo.2014.2661