miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma

  • Authors:
    • Panagiotis G. Adamopoulos
    • Christos K. Kontos
    • Stamatia-Maria Rapti
    • Iordanis N. Papadopoulos
    • Andreas Scorilas
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  • Published online on: November 24, 2014     https://doi.org/10.3892/ijo.2014.2775
  • Pages: 849-859
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Abstract

Colorectal adenocarcinoma constitutes the most frequent form of colorectal cancer and a serious cause of cancer‑related deaths. The expression of multiple miRNAs, including miR-224, is deregulated in colorectal adenocarcinoma. The aim of this study was the investigation of the prognostic value of miR-224 in colorectal adenocarcinoma. For this purpose, total RNA was isolated from 115 colorectal adenocarcinomas and 66 adjacent non-cancer mucosae. Total RNA (2 µg) was polyadenylated and reverse transcribed. A quantitative PCR method based on SYBR‑Green chemistry was developed and applied for the quantification of miR-224 levels, followed by extensive biostatistical analysis. miR-224 levels in malignant colorectal adenocarcinomas ranged between 1.81 and 187.75 RQU (miR-224 copies/1,000 SNORD48 copies) with a median of 34.27, and were significantly elevated, compared to miR-224 levels in adjacent non-cancer mucosae (p<0.001). Enhanced miR-224 expression constitutes a rather strong prognosticator in colorectal adenocarcinoma, predicting short-term relapse and poor overall survival in these patients (p=0.012 and p=0.005, respectively), independent of established clinicopathological parameters. In conclusion, miR-224 is significantly upregulated in malignant colorectal tumors compared to adjacent non-cancer mucosae, and its enhanced expression constitutes an independent predictor of short-term relapse and poor overall survival in colorectal adenocarcinoma patients.
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February-2015
Volume 46 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Adamopoulos PG, Kontos CK, Rapti S, Papadopoulos IN and Scorilas A: miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma. Int J Oncol 46: 849-859, 2015
APA
Adamopoulos, P.G., Kontos, C.K., Rapti, S., Papadopoulos, I.N., & Scorilas, A. (2015). miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma. International Journal of Oncology, 46, 849-859. https://doi.org/10.3892/ijo.2014.2775
MLA
Adamopoulos, P. G., Kontos, C. K., Rapti, S., Papadopoulos, I. N., Scorilas, A."miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma". International Journal of Oncology 46.2 (2015): 849-859.
Chicago
Adamopoulos, P. G., Kontos, C. K., Rapti, S., Papadopoulos, I. N., Scorilas, A."miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma". International Journal of Oncology 46, no. 2 (2015): 849-859. https://doi.org/10.3892/ijo.2014.2775