Open Access

Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells

  • Authors:
    • Alessandro Salvi
    • Italia Bongarzone
    • Lia Ferrari
    • Edoardo Abeni
    • Bruna Arici
    • Maida De Bortoli
    • Sabrina Scuri
    • Daniela Bonini
    • Ilaria Grossi
    • Anna Benetti
    • Gianluca Baiocchi
    • Nazario Portolani
    • Giuseppina De Petro
  • View Affiliations

  • Published online on: March 9, 2015     https://doi.org/10.3892/ijo.2015.2923
  • Pages: 1901-1912
  • Copyright: © Salvi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. We have previously reported that LASP-1 is a downstream protein of the urokinase type plasminogen activator (uPA). Here we investigated the role of LASP-1 in HCC by a molecular and biological characterization of LASP-1 expression in human HCC specimens and in cultured HCC cells. We determined the LASP-1 mRNA expression levels in 55 HCC cases with different hepatic background disease. We identified 3 groups of patients with high, equal or low LASP-1 mRNA levels in HCC tissues compared to the peritumoral (PT) tissues. In particular we found that i) the HCCs displayed a higher LASP-1 mRNA level in HCC compared to PT tissues; ii) the expression levels of LASP-1 mRNA in female HCCs were significantly higher compared to male HCCs; iii) the cirrhotic HCCs displayed a higher LASP-1 mRNA. Further, the biological characterization of the ectopic LASP-1 overexpression in HCC cells, using MALDI-TOF mass spectrometer on the LASP-1 co-immunoprecipitated fractions, displayed vimentin as a novel putative partner of LASP-1. Our results suggest that LASP-1 mRNA overexpression may be mainly implicated in female HCCs and cirrhotic HCCs; and that LASP1 may play its role with vimentin in HCC cells.
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May-2015
Volume 46 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Salvi A, Bongarzone I, Ferrari L, Abeni E, Arici B, De Bortoli M, Scuri S, Bonini D, Grossi I, Benetti A, Benetti A, et al: Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. Int J Oncol 46: 1901-1912, 2015
APA
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M. ... De Petro, G. (2015). Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. International Journal of Oncology, 46, 1901-1912. https://doi.org/10.3892/ijo.2015.2923
MLA
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., De Petro, G."Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells". International Journal of Oncology 46.5 (2015): 1901-1912.
Chicago
Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., De Petro, G."Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells". International Journal of Oncology 46, no. 5 (2015): 1901-1912. https://doi.org/10.3892/ijo.2015.2923