Open Access

Characterization of glioma stem-like cells from human glioblastomas

  • Authors:
    • Shun Yamamuro
    • Yutaka Okamoto
    • Emiko Sano
    • Yushi Ochiai
    • Akiyoshi Ogino
    • Takashi Ohta
    • Hiroyuki Hara
    • Takuya Ueda
    • Tomohiro Nakayama
    • Atsuo Yoshino
    • Yoichi Katayama
  • View Affiliations

  • Published online on: May 7, 2015     https://doi.org/10.3892/ijo.2015.2992
  • Pages: 91-96
  • Copyright: © Yamamuro et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Glioma stem-like cells (GSCs) could have potential for tumorigenesis, treatment resistance, and tumor recurrence (GSC hypothesis). However, the mechanisms underlying such potential has remained elusive and few ultrastructural features of the cells have been reported in detail. We therefore undertook observations of the antigenic characteristics and ultrastructural features of GSCs isolated from human glioblastomas. Tumor spheres formed by variable numbers of cells, exhibiting a variable appearance in both their size and shape, were frequently seen in GSCs expressing the stem cell surface markers CD133 and CD15. Increased cell nucleus atypia, mitochondria, rough endoplasmic reticulum, coated vesicles, and microvilli, were noted in the GSCs. Furthermore, cells at division phases and different phases of the apoptotic process were occasionally observed. These findings could imply that GSCs have certain relations with human neural stem cells (NSCs) but are primitively different from undifferentiated NSCs. The data may provide support for the GSC hypothesis, and also facilitate the establishment of future glioblastoma treatments targeting GSCs.
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July-2015
Volume 47 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Yamamuro S, Okamoto Y, Sano E, Ochiai Y, Ogino A, Ohta T, Hara H, Ueda T, Nakayama T, Yoshino A, Yoshino A, et al: Characterization of glioma stem-like cells from human glioblastomas. Int J Oncol 47: 91-96, 2015
APA
Yamamuro, S., Okamoto, Y., Sano, E., Ochiai, Y., Ogino, A., Ohta, T. ... Katayama, Y. (2015). Characterization of glioma stem-like cells from human glioblastomas. International Journal of Oncology, 47, 91-96. https://doi.org/10.3892/ijo.2015.2992
MLA
Yamamuro, S., Okamoto, Y., Sano, E., Ochiai, Y., Ogino, A., Ohta, T., Hara, H., Ueda, T., Nakayama, T., Yoshino, A., Katayama, Y."Characterization of glioma stem-like cells from human glioblastomas". International Journal of Oncology 47.1 (2015): 91-96.
Chicago
Yamamuro, S., Okamoto, Y., Sano, E., Ochiai, Y., Ogino, A., Ohta, T., Hara, H., Ueda, T., Nakayama, T., Yoshino, A., Katayama, Y."Characterization of glioma stem-like cells from human glioblastomas". International Journal of Oncology 47, no. 1 (2015): 91-96. https://doi.org/10.3892/ijo.2015.2992