miR-506 regulates breast cancer cell metastasis by targeting IQGAP1

  • Authors:
    • Guang Sun
    • Yanxi Liu
    • Keren Wang
    • Zheli Xu
  • View Affiliations

  • Published online on: September 14, 2015     https://doi.org/10.3892/ijo.2015.3161
  • Pages: 1963-1970
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Abstract

MicroRNA (miRNA or miR)-506 is a novel miRNA related to the survival of breast cancer patients. However, the mechanism underlying miRNA-506 involvement in breast carcinogenesis remains unclear. In the present study, we found that miR-506 was downregulated in human breast malignant tissues and breast cancer cell lines by RT-qPCR analysis, and the expression level of miR-506 was decreased with the increasing of tumor stage. Subsequently, gain-of-function and loss-of-function experiments were performed in vitro, and the results from MTT assay, Transwell-Matrigel invasion assay and cell adhesion assay revealed that miR-506 suppresses cell proliferation, invasion and adhesion of breast cancer cells. Luciferase reporter assay revealed that IQ motif containing GTPase activating protein 1 (IQGAP1) is a direct target of miR-506. miR-506 represses the expression of IQGAP1 and its downstream extracellular signal regulated kinase (ERK) mitogen-activated protein kinase (MAPK) signaling pathways, as demonstrated by the RT-qPCR and western blot analysis. Furthermore, we found that IQGAP1 rescues the effect of miR-506 on cell proliferation, invasion, adhesion, and the activation of ERK MAPK signaling. In conclusion, the present study is the first to provide evidence that miR-506 acts as a tumor suppressor, at least partially, by directly downregulating IQGAP1 in breast cancer cells. The miR-506/IQGAP1/ERK pathway may be a novel therapeutic target in breast cancer.
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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sun G, Liu Y, Wang K and Xu Z: miR-506 regulates breast cancer cell metastasis by targeting IQGAP1. Int J Oncol 47: 1963-1970, 2015
APA
Sun, G., Liu, Y., Wang, K., & Xu, Z. (2015). miR-506 regulates breast cancer cell metastasis by targeting IQGAP1. International Journal of Oncology, 47, 1963-1970. https://doi.org/10.3892/ijo.2015.3161
MLA
Sun, G., Liu, Y., Wang, K., Xu, Z."miR-506 regulates breast cancer cell metastasis by targeting IQGAP1". International Journal of Oncology 47.5 (2015): 1963-1970.
Chicago
Sun, G., Liu, Y., Wang, K., Xu, Z."miR-506 regulates breast cancer cell metastasis by targeting IQGAP1". International Journal of Oncology 47, no. 5 (2015): 1963-1970. https://doi.org/10.3892/ijo.2015.3161