Sulforaphane inhibits hypoxia-induced HIF-1α and VEGF expression and migration of human colon cancer cells

  • Authors:
    • Dong Hwan Kim
    • Bokyung Sung
    • Yong Jung Kang
    • Seong Yeon Hwang
    • Min Jeong Kim
    • Jeong-Hyun Yoon
    • Eunok Im
    • Nam Deuk Kim
  • View Affiliations

  • Published online on: October 13, 2015     https://doi.org/10.3892/ijo.2015.3200
  • Pages: 2226-2232
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Abstract

The effects of sulforaphane (a natural product commonly found in broccoli) was investigated on hypoxia inducible factor-1α (HIF-1α) expression in HCT116 human colon cancer cells and AGS human gastric cancer cells. We found that hypoxia-induced HIF-1α protein expression in HCT116 and AGS cells, while treatment with sulforaphane markedly and concentration-dependently inhibited HIF-1α expression in both cell lines. Treatment with sulforaphane inhibited hypoxia-induced vascular endothelial growth factor (VEGF) expression in HCT116 cells. Treatment with sulforaphane modulated the effect of hypoxia on HIF-1α stability. However, degradation of HIF-1α by sulforaphane was not mediated through the 26S proteasome pathway. We also found that the inhibition of HIF-1α by sulforaphane was not mediated through AKT and extracellular signal-regulated kinase phosphorylation under hypoxic conditions. Finally, hypoxia-induced HCT116 cell migration was inhibited by sulforaphane. These data suggest that sulforaphane may inhibit human colon cancer progression and cancer cell angiogenesis by inhibiting HIF-1α and VEGF expression. Taken together, these results indicate that sulforaphane is a new and potent chemopreventive drug candidate for treating patients with human colon cancer.
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December-2015
Volume 47 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim DH, Sung B, Kang YJ, Hwang SY, Kim MJ, Yoon J, Im E and Kim ND: Sulforaphane inhibits hypoxia-induced HIF-1α and VEGF expression and migration of human colon cancer cells. Int J Oncol 47: 2226-2232, 2015
APA
Kim, D.H., Sung, B., Kang, Y.J., Hwang, S.Y., Kim, M.J., Yoon, J. ... Kim, N.D. (2015). Sulforaphane inhibits hypoxia-induced HIF-1α and VEGF expression and migration of human colon cancer cells. International Journal of Oncology, 47, 2226-2232. https://doi.org/10.3892/ijo.2015.3200
MLA
Kim, D. H., Sung, B., Kang, Y. J., Hwang, S. Y., Kim, M. J., Yoon, J., Im, E., Kim, N. D."Sulforaphane inhibits hypoxia-induced HIF-1α and VEGF expression and migration of human colon cancer cells". International Journal of Oncology 47.6 (2015): 2226-2232.
Chicago
Kim, D. H., Sung, B., Kang, Y. J., Hwang, S. Y., Kim, M. J., Yoon, J., Im, E., Kim, N. D."Sulforaphane inhibits hypoxia-induced HIF-1α and VEGF expression and migration of human colon cancer cells". International Journal of Oncology 47, no. 6 (2015): 2226-2232. https://doi.org/10.3892/ijo.2015.3200