Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion

  • Authors:
    • Myung-Ha Song
    • Ye-Rin Kim
    • Jun-Won Lee
    • Chang-Hun Lee
    • Sang-Yull Lee
  • View Affiliations

  • Published online on: November 24, 2015     https://doi.org/10.3892/ijo.2015.3264
  • Pages: 569-576
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Abstract

The cancer/testis antigen NY-SAR-35 is aberrantly expressed in various cancer tissues and cancer cell lines but not in normal tissues except for the testis. A previous study demonstrated that the expression of NY-SAR-35 is activated by hypomethylation in cancer cells. However, the functions of this antigen remain unexplored. In the present study, we investigated the role of NY-SAR‑35 in human embryonic kidney (HEK) 293 cells using exogenous expression system of the gene. NY-SAR‑35 was predominantly expressed at the cytoplasm and was mainly observed in spermatogonia and spermatocytes. Expression of NY-SAR-35 in stable HEK293 transfectant clones was 2-fold higher than the control cells promoting cell growth and proliferation. NY-SAR-35 overexpression also enhanced cell migration and invasion ~2-fold and 4-fold more than the control, respectively. In contrast, small interfering RNA-mediated knockdown of NY-SAR-35 suppressed cell proliferation, migration, and invasion in HEK293 stable transfectants. We concluded that NY-SAR-35 as a cancer/testis antigen enhanced cell proliferation and invasion.

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February 2016
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APA
Song, M., Kim, Y., Lee, J., Lee, C., & Lee, S. (2016). Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion. International Journal of Oncology, 48, 569-576. https://doi.org/10.3892/ijo.2015.3264
MLA
Song, M., Kim, Y., Lee, J., Lee, C., Lee, S."Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion". International Journal of Oncology 48.2 (2016): 569-576.
Chicago
Song, M., Kim, Y., Lee, J., Lee, C., Lee, S."Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion". International Journal of Oncology 48, no. 2 (2016): 569-576. https://doi.org/10.3892/ijo.2015.3264