Open Access

In vivo monitoring of CD44+ cancer stem-like cells by γ-irradiation in breast cancer

  • Authors:
    • Mi Hyun Kim
    • Min Hwan Kim
    • Kwang Seok Kim
    • Myung-Jin Park
    • Jae-Hoon Jeong
    • Seung Woo Park
    • Young Hoon Ji
    • Kwang Il Kim
    • Tae Sup Lee
    • Phil Youl Ryu
    • Joo Hyun Kang
    • Yong Jin Lee
  • View Affiliations

  • Published online on: April 18, 2016     https://doi.org/10.3892/ijo.2016.3493
  • Pages: 2277-2286
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

There is increasing evidence that cancer contains cancer stem cells (CSCs) that are capable of regenerating a tumor following chemotherapy or radiotherapy. CD44 and CD133 are used to identify CSCs. This study investigated non-invasive in vivo monitoring of CD44-positive cancer stem-like cells in breast cancer by γ-irradiation using molecular image by fusing the firefly luciferase (fLuc) gene with the CD44 promoter. We generated a breast cancer cell line stably expressing fLuc gene by use of recombinant lentiviral vector controlled by CD44 promoter (MCF7-CL). Irradiated MCF7-CL spheres showed upregulated expression of CD44 and CD133, by immunofluorescence and flow cytometry. Also, gene expression levels of CSCs markers in irradiated spheres were clearly increased. CD44+ CSCs increased fLuc expression and tumor growth in vivo and in vitro. When MCF7-CL was treated with siCD44 and irradiated, CD44 expression was inhibited and cell survival ratio was decreased. MCF7-CL subsets were injected into the mice and irradiated by using a cobalt-60 source. Then, in vivo monitoring was performed to observe the bioluminescence imaging (BLI). When breast cancer was irradiated, relative BLI signal was increased, but tumor volume was decreased compared to non-irradiated tumor. These results indicate that increased CD44 expression, caused by general feature of CSCs by irradiation and sphere formation, can be monitored by using bioluminescence imaging. This system could be useful to evaluate CD44- expressed CSCs in breast cancer by BLI in vivo as well as in vitro for radiotherapy.
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June-2016
Volume 48 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Kim MH, Kim MH, Kim KS, Park M, Jeong J, Park SW, Ji YH, Kim KI, Lee TS, Ryu PY, Ryu PY, et al: In vivo monitoring of CD44+ cancer stem-like cells by γ-irradiation in breast cancer. Int J Oncol 48: 2277-2286, 2016
APA
Kim, M.H., Kim, M.H., Kim, K.S., Park, M., Jeong, J., Park, S.W. ... Lee, Y.J. (2016). In vivo monitoring of CD44+ cancer stem-like cells by γ-irradiation in breast cancer. International Journal of Oncology, 48, 2277-2286. https://doi.org/10.3892/ijo.2016.3493
MLA
Kim, M. H., Kim, M. H., Kim, K. S., Park, M., Jeong, J., Park, S. W., Ji, Y. H., Kim, K. I., Lee, T. S., Ryu, P. Y., Kang, J. H., Lee, Y. J."In vivo monitoring of CD44+ cancer stem-like cells by γ-irradiation in breast cancer". International Journal of Oncology 48.6 (2016): 2277-2286.
Chicago
Kim, M. H., Kim, M. H., Kim, K. S., Park, M., Jeong, J., Park, S. W., Ji, Y. H., Kim, K. I., Lee, T. S., Ryu, P. Y., Kang, J. H., Lee, Y. J."In vivo monitoring of CD44+ cancer stem-like cells by γ-irradiation in breast cancer". International Journal of Oncology 48, no. 6 (2016): 2277-2286. https://doi.org/10.3892/ijo.2016.3493