Tissue microRNAs as predictive markers for gastric cancer patients undergoing palliative chemotherapy

  • Authors:
    • David Smid
    • Vlastimil Kulda
    • Kristyna Srbecka
    • Dasa Kubackova
    • Jan Dolezal
    • Ondrej Daum
    • Radek Kucera
    • Ondrej Topolcan
    • Vladislav Treska
    • Tomas Skalicky
    • Martin Pesta
  • View Affiliations

  • Published online on: April 12, 2016     https://doi.org/10.3892/ijo.2016.3484
  • Pages: 2693-2703
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Abstract

MicroRNAs have the potential to become valuable predictive markers for gastric cancer. Samples of biopsy tissue, routinely taken from gastric cancer patients undergoing palliative chemotherapy, constitute suitable material for microRNA profiling with the aim of predicting the effect of chemotherapy. Our study group consisted of 54 patients, all of whom underwent palliative chemotherapy based on 5-fluorouracil (5-FU) or 5-FU in combination with platinum derivatives between 2000 and 2013. The expression of 29 selected microRNAs and genes BRCA1, ERCC1, RRM1 and TS, in gastric cancer tissue macrodissected from FFPE tissue samples, was measured by quantitative RT-PCR. The relationship between gene expression levels and time to progression (TTP) and overall survival (OS) was analysed. From the set of the 29 microRNAs of interest, we found high expression of miR-150, miR-342-3p, miR-181b, miR-221, miR-224 and low levels of miR-520h relate to shorter TTP. High levels of miR-150, miR-192, miR-224, miR-375 and miR-342-3p related to shorter OS. In routinely available FFPE tissue samples, we found 6 miRNAs with a relation to TTP, which may serve as predictors of the effectiveness of palliative treatment in gastric cancer patients. These miRNAs could also help in deciding whether to indicate palliative chemotherapy.
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June-2016
Volume 48 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Smid D, Kulda V, Srbecka K, Kubackova D, Dolezal J, Daum O, Kucera R, Topolcan O, Treska V, Skalicky T, Skalicky T, et al: Tissue microRNAs as predictive markers for gastric cancer patients undergoing palliative chemotherapy. Int J Oncol 48: 2693-2703, 2016
APA
Smid, D., Kulda, V., Srbecka, K., Kubackova, D., Dolezal, J., Daum, O. ... Pesta, M. (2016). Tissue microRNAs as predictive markers for gastric cancer patients undergoing palliative chemotherapy. International Journal of Oncology, 48, 2693-2703. https://doi.org/10.3892/ijo.2016.3484
MLA
Smid, D., Kulda, V., Srbecka, K., Kubackova, D., Dolezal, J., Daum, O., Kucera, R., Topolcan, O., Treska, V., Skalicky, T., Pesta, M."Tissue microRNAs as predictive markers for gastric cancer patients undergoing palliative chemotherapy". International Journal of Oncology 48.6 (2016): 2693-2703.
Chicago
Smid, D., Kulda, V., Srbecka, K., Kubackova, D., Dolezal, J., Daum, O., Kucera, R., Topolcan, O., Treska, V., Skalicky, T., Pesta, M."Tissue microRNAs as predictive markers for gastric cancer patients undergoing palliative chemotherapy". International Journal of Oncology 48, no. 6 (2016): 2693-2703. https://doi.org/10.3892/ijo.2016.3484