Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma

  • Authors:
    • Guang-Lin Song
    • Cong-Cong Jin
    • Wei Zhao
    • Yi Tang
    • Ya-Lan Wang
    • Ming Li
    • Ming Xiao
    • Xian Li
    • Qing-Shu Li
    • Xiao Lin
    • Wen-Wen Chen
    • Jing Kuang
  • View Affiliations

  • Published online on: May 27, 2016     https://doi.org/10.3892/ijo.2016.3539
  • Pages: 646-656
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Abstract

Arginine-specific ADP-ribosytransferases 1 (ART1) is able to modify the arginine of specific proteins by mono-ADP-ribosylation. We previously reported that the expression of ART1 in human colon adenocarcinoma tissues was higher than in adjacent tissues. Herein, we primarily revealed that ART1 could regulate the epithelial-mesenchymal transition (EMT) and, therefore, the development of colon carcinoma. In CT26 cells, which overexpressed ART1 by lentiviral transfection, the following were promoted: alterations of spindle-like non-polarization, expression of EMT inducers and mesenchymal markers, migration, invasion and adhesion. However, epithelial marker expression was decreased. Correspondingly, knockdown of ART1 in CT26 cells had the opposite effects. The effect of ART1 on EMT and carcinoma metastasis was also verified in a liver metastasis model of BALB/c mice. To further explore the molecular mechanism of ART1 in EMT, CT26 cells were treated with several specific inhibitors and gene silencing. Our data suggest that ART1 could regulate EMT by regulating the RhoA/ROCK1/AKT/β-catenin pathway and its downstream factors (snail1, vimentin, N-cadherin and E-cadherin) and that it therefore plays an important role in the progression of colon carcinoma.
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August-2016
Volume 49 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Song G, Jin C, Zhao W, Tang Y, Wang Y, Li M, Xiao M, Li X, Li Q, Lin X, Lin X, et al: Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma. Int J Oncol 49: 646-656, 2016
APA
Song, G., Jin, C., Zhao, W., Tang, Y., Wang, Y., Li, M. ... Kuang, J. (2016). Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma. International Journal of Oncology, 49, 646-656. https://doi.org/10.3892/ijo.2016.3539
MLA
Song, G., Jin, C., Zhao, W., Tang, Y., Wang, Y., Li, M., Xiao, M., Li, X., Li, Q., Lin, X., Chen, W., Kuang, J."Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma". International Journal of Oncology 49.2 (2016): 646-656.
Chicago
Song, G., Jin, C., Zhao, W., Tang, Y., Wang, Y., Li, M., Xiao, M., Li, X., Li, Q., Lin, X., Chen, W., Kuang, J."Regulation of the RhoA/ROCK/AKT/β-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma". International Journal of Oncology 49, no. 2 (2016): 646-656. https://doi.org/10.3892/ijo.2016.3539