ANALYSIS OF INTRACELLULAR RETENTION OF MORPHOLINYL ANTHRACYCLINES IN MULTIDRUG-RESISTANT CANCER-CELLS BY INTERACTIVE LASER CYTOMETRY

  • Authors:
    • DHM LAU
    • GE DURAN
    • BI SIKIC
  • View Affiliations

  • Published online on: December 1, 1994     https://doi.org/10.3892/ijo.5.6.1273
  • Pages: 1273-1277
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Abstract

Interactive laser cytometry was applied to measure intracellular fluorescence of doxorubicin (DOX) accumulation in a uterine sarcoma cell line, MES-SA and a series of multidrug resistant sublines, Dx0.3, Dx1 and Dx5. Exposure of each cell line to 10 mu M DOX for 2 h resulted in an intracellular fluorescent level directly correlated to its sensitivity to the drug but inversely related to its cellular P-glycoprotein (P-gp) level. The morpholinyl anthracyclines, methoxymorpholinyl DOX (MMDX) and morpholinyl DOX (MRA), were equally highly cytotoxic against the multidrug sensitive and resistant cancer cells. After exposure to 10 mu M of MMDX or MRA for 2 h, the multidrug resistant cells, Dx5, retained as much intracellular fluorescence as the multidrug sensitive cells, MES-SA. In the resistant cells, the intracellular fluorescence of MMDX or MRA was 8 fold higher than that of DOX. Interactive laser. cytometer is a useful fool for screening cancer cells with the MDR phenotype and for identifying new anthracyclines effective against drug resistant malignancies.

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December 1994
Volume 5 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
LAU D, DURAN G and SIKIC B: ANALYSIS OF INTRACELLULAR RETENTION OF MORPHOLINYL ANTHRACYCLINES IN MULTIDRUG-RESISTANT CANCER-CELLS BY INTERACTIVE LASER CYTOMETRY. Int J Oncol 5: 1273-1277, 1994
APA
LAU, D., DURAN, G., & SIKIC, B. (1994). ANALYSIS OF INTRACELLULAR RETENTION OF MORPHOLINYL ANTHRACYCLINES IN MULTIDRUG-RESISTANT CANCER-CELLS BY INTERACTIVE LASER CYTOMETRY. International Journal of Oncology, 5, 1273-1277. https://doi.org/10.3892/ijo.5.6.1273
MLA
LAU, D., DURAN, G., SIKIC, B."ANALYSIS OF INTRACELLULAR RETENTION OF MORPHOLINYL ANTHRACYCLINES IN MULTIDRUG-RESISTANT CANCER-CELLS BY INTERACTIVE LASER CYTOMETRY". International Journal of Oncology 5.6 (1994): 1273-1277.
Chicago
LAU, D., DURAN, G., SIKIC, B."ANALYSIS OF INTRACELLULAR RETENTION OF MORPHOLINYL ANTHRACYCLINES IN MULTIDRUG-RESISTANT CANCER-CELLS BY INTERACTIVE LASER CYTOMETRY". International Journal of Oncology 5, no. 6 (1994): 1273-1277. https://doi.org/10.3892/ijo.5.6.1273