Rb knockdown accelerates bladder cancer progression through E2F3 activation

  • Authors:
    • Jiang-Ping Wang
    • Yong Jiao
    • Cheng-Yuan Wang
    • Zhi-Bin Xu
    • Bo Zhang
  • View Affiliations

  • Published online on: December 6, 2016     https://doi.org/10.3892/ijo.2016.3791
  • Pages: 149-160
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Abstract

Bladder cancer is one of the most common cancers diagnosed in the world and leads to significant mortality and morbidity among affected patients. The retinoblastoma (Rb) protein is a main tumor suppressor, controlling cellular responses to potentially oncogenic stimulation. E2F3 was invariably disrupted in different human cancers for its central role in the control of cellular proliferation. Here, we investigated how Rb is integrated to control bladder cancer progression through E2F3 and p53 regulation. The results exhibit that Rb expression is lower in patients with bladder tumor, while E2F3 level is high. Rb knockdown enhanced bladder tumor cell proliferation and migration, aggravated with p53 silence. Interestingly, Rb silence results in E2F3, Myc and mTOR signaling pathway activation, contributing to bladder cancer cell proliferation and apoptosis suppression mainly through caspase-3 inhibition in vitro and in vivo. Immunohistochemical analysis revealed that Rb is highly expressed in normal bladder cells, but was repressed in tumor tissues of the bladder completely, suggesting a possible role of Rb as a tumor suppressor.
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January-2017
Volume 50 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang J, Jiao Y, Wang C, Xu Z and Zhang B: Rb knockdown accelerates bladder cancer progression through E2F3 activation. Int J Oncol 50: 149-160, 2017
APA
Wang, J., Jiao, Y., Wang, C., Xu, Z., & Zhang, B. (2017). Rb knockdown accelerates bladder cancer progression through E2F3 activation. International Journal of Oncology, 50, 149-160. https://doi.org/10.3892/ijo.2016.3791
MLA
Wang, J., Jiao, Y., Wang, C., Xu, Z., Zhang, B."Rb knockdown accelerates bladder cancer progression through E2F3 activation". International Journal of Oncology 50.1 (2017): 149-160.
Chicago
Wang, J., Jiao, Y., Wang, C., Xu, Z., Zhang, B."Rb knockdown accelerates bladder cancer progression through E2F3 activation". International Journal of Oncology 50, no. 1 (2017): 149-160. https://doi.org/10.3892/ijo.2016.3791