miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells

  • Authors:
    • Eun-Ae Kim
    • Tae Ghab Kim
    • Eon-Gi Sung
    • In-Hwan Song
    • Joo-Young Kim
    • Kyung-Oh Doh
    • Tae-Jin Lee
  • View Affiliations

  • Published online on: January 17, 2017     https://doi.org/10.3892/ijo.2017.3851
  • Pages: 984-992
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Abstract

MicroRNA (miR) can exert various biological functions by targeting oncogenes or tumor suppressor genes in numerous human malignancies. Recent evidence has shown that miR-148a increases the drug sensitivity of various cancer cells. Herein, we show that ectopic expression of miR-148a induces apoptosis, reduces clonogenicity, and increases the sensitivity to TRAIL and cisplatin in renal cancer cells. The luciferase reporter assay showed that miR-148a negatively regulated ras-related protein 14 (Rab14) expression by binding to the miR-148a binding site in the 3' untranslated region (3'UTR) of Rab14. Rab14-specific siRNA-induced downregulation of Rab14 increases the sensitivity to cisplatin, while forced expression of Rab14 lacking 3'-UTR abrogated the pro-apoptotic function of miR-148a in renal cancer cells. These findings suggest that miR-148a acts as a tumor suppressor and holds great potential for renal cancer therapy by directly targeting Rab14.
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March-2017
Volume 50 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim E, Kim TG, Sung E, Song I, Kim J, Doh K and Lee T: miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells. Int J Oncol 50: 984-992, 2017
APA
Kim, E., Kim, T.G., Sung, E., Song, I., Kim, J., Doh, K., & Lee, T. (2017). miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells. International Journal of Oncology, 50, 984-992. https://doi.org/10.3892/ijo.2017.3851
MLA
Kim, E., Kim, T. G., Sung, E., Song, I., Kim, J., Doh, K., Lee, T."miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells". International Journal of Oncology 50.3 (2017): 984-992.
Chicago
Kim, E., Kim, T. G., Sung, E., Song, I., Kim, J., Doh, K., Lee, T."miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells". International Journal of Oncology 50, no. 3 (2017): 984-992. https://doi.org/10.3892/ijo.2017.3851